Optimiranje uvjeta analize S-fenilmerkapturne kiseline u mokraći primjenom vezanog sustava plinski kromatograf-spektrometar masa

master's thesis

• Title (english): Optimising the conditions for S-phenylmercapturic acid analysis in urine using gas chromatography-mass spectrometry
• Author: Doroteja Cindrić
• Mentor: Irena Brčić Karačonji (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Ivica Đilović (član povjerenstva)
• Committee member: Đani Škalamera (član povjerenstva)
• Committee member: Adriana Kenđel (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Benzen je aromatski spoj s kancerogenim svojstvima kojem su ljudi, osim profesionalno, izloženi putem cigaretnog dima ili ispušnih plinova motornih vozila. S-Fenilmerkapturna kiselina (S-PMA) smatra se specifičnim pokazateljem izloženosti benzenu kod opće populacije i pri profesionalnoj izloženosti. Cilj ovog istraživanja bio je optimizirati uvjete ekstrakcije SPMA iz mokraće te razviti i validirati osjetljivu i specifičnu analitičku metodu za određivanje masene koncentracije S-PMA u mokraći primjenom vezanog sustava plinski kromatografspektrometar masa. Predložena metoda je pokazala dobru linearnost (R2>0,9996) u ispitivanom koncentracijskom rasponu (0,5 – 100 μg L-1), preciznost (RSD<7 %), točnost (>95 %) i osjetljivost (granica detekcije: 0,03 μg L-1i granica kvantifikacije: 0,09 μg L-1). Metoda je primijenjena za određivanje masene koncentracije S-PMA u mokraći 20 ispitanika koji nisu bili profesionalno izloženi benzenu. Pušači (N=10) su imali značajno višu koncentraciju S-PMA u mokraći u usporedbi s nepušačima (N=10).

Abstract (english)

Benzene is an aromatic compound with carcinogenic properties to which people, except in professional settings, are exposed by cigarette smoke or motor vehicle exhaust. Sphenylmercapturic acid (S-PMA) is considered a specific marker of benzene exposure in the general and occupationally exposed population. The aim of this study was to optimise the conditions of S-PMA extraction from urine and to develop and validate a sensitive and specific analytical method for determining the mass concentration of S-PMA in urine using gas chromatography-mass spectrometry. The proposed method showed good linearity (R2> 0,9996) in the examined concentration range (0,5 – 100 μg L-1), good precision (RSD <7%), accuracy (> 95%) and sensitivity (limit of detection: 0,03 μg L-1 1 and limit of quantification: 0,09 μg L1). The method was applied to determine the mass concentration of S-PMA in the urine of 20 subjects who were not occupationally exposed to benzene. Smokers (N = 10) had a significantly higher concentration of S-PMA in urine compared to non-smokers (N = 10).

• Keywords: benzen; ekstrakcija; mokraća; plinski kromatograf; spektrometar masa; S-PMA
• Keywords (english): benzene; extraction; gas chromatograph; mass spectrometer; S-PMA; urine
• Language: croatian
• URN:NBN: urn:nbn:hr:217:466382
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-29
• Created on: 2021-10-18 09:55:09