Morfološka i genska raznolikost vrsta roda Apodemus na području Parka prirode Medvednica

master's thesis

• Title (english): Morphological and genetic diversity of Apodemus species in Medvednica Nature Park
• Author: Dora Čuljak
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (predsjednik povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rod šumskih miševa, Apodemus Kaup, 1829 u Hrvatskoj broji 5 vrsta, od kojih su najčešće Apodemus flavicollis (Melchior, 1834) i Apodemus sylvaticus (Linnaeus, 1758). Ove dvije vrste su morfološki ekološki te ponašanjem vrlo slične. Često žive simpatrijski, te zauzimaju gotovo iste areale. Obje su prisutne na području Parka prirode Medvednica, a uz njih tu nalazimo i lako prepoznatljivu vrstu Apodemus agrarius (Pallas, 1771). Cilj ovog istraživanja bio je uz pomoć gena za citokrom b dobiti uvid u srodstvene odnose i gensku raznolikost ovih vrsta. Također, cilj je bio usporediti njihove morfometrijske značajke pri čemu sam probala pronaći metodu za raspoznavanje i determinaciju vrsta A. flavicollis i A. sylvaticus na temelju vanjskih mjera tijela. Filogenetska rekonstrukcija metodama najveće parsimonije i najveće vjerojatnosti te broj dijagnostičkih mjesta ukazali su na prisutnost kriptičke raznolikosti i jasnu odvojenost istraživanih jedinki u dvije vrste: A. flavicollis te Apodemus sp. Niti jedna analizirana jedinka ne pripada vrsti A. sylvaticus. Visoka genska razolikost istraživanih skupina upućuje na stabilnost i dugo postojanje ovih populacija na području Medvednice, što ide u prilog hipotezi o postojanju glacijalnog refugija na području jugoistočne Europe. Za pouzdaniju detereminaciju vrsta putem njihove morfometrije potrebno je ponoviti test na većem uzorku. Također je potrebna taksonomska revizija roda Apodemus u Hrvatskoj.

Abstract (english)

So far 5 species of field mice in the genus Apodemus Kaup, 1829 have been recorded in Croatia. The most common ones are Apodemus flavicollis (Melchior, 1834) and Apodemus sylvaticus (Linnaeus, 1758). These two species are morphologically, ecologically, and behaviorally very similar, often having sympatric distribution. Both are present in the area of Nature Park Medvednica, along with easily discernible species Apodemus agrarius (Pallas, 1771). Aim of this research was to gain insight into their phylogeny and genetic diversity through cytochrome b genes. Their morphometrics were compared as well to find a method for discrimination of species A. flavicollis and A. sylvaticus based on body measurements. Phylogenetic reconstruction using maximum parsimony and maximum likelihood, as well as the number of sites od fixed differences in DNA have shown a clear separation of tested individuals into two groups: A. flavicollis and Apodemus sp., while no individual was identified as A. sylvaticus. High genetic diversity of studied taxa signifies stability and long presence of these population on Medvednica mountain, which is in accordance with the hypothesis of glacial refugii in the southeastern Europe. To get a reliable discriminating morphometrical characteristic, research should be repeated on a bigger sample. A taxonomic revision of genus Apodemus in Croatia is needed.

• Keywords: morfometrija; filogenetika; citokrom b; A. flavicollis; A. sylvaticus; A. agrarius
• Keywords (english): morphometrics; phylogenetics; cytochrome b; A. flavicollis; A. sylvaticus; A. agrarius
• Language: croatian
• URN:NBN: urn:nbn:hr:217:437353
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-11-28
• Created on: 2022-03-16 10:29:52