Primjena mikroskopije prilikom istraživanja bubrežnih kamenaca

master's thesis

• Title (english): Use of microscopy in renal stones research
• Author: Sanda Horvatić
• Mentor: Vladimir Bermanec (mentor)
• Committee member: Vladimir Bermanec (predsjednik povjerenstva)
• Committee member: Jasna Hrenović (član povjerenstva)
• Committee member: Jasenka Sremac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Geology); Zagreb
• Defense date and country: 2015-03-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Geology

Abstract

Analizirano je sedam uzoraka bubrežnih kamenaca prikupljenih od bolesnika na području grada Zagreba i grada Osijeka. Korištene su metode rentgenske difrakcije na prahu (XRD), pretražne elektronske mikroskopije (SEM) i mikroskopije atomskih sila (AFM). Rentgenskom analizom dobivene su informacije o kemijskom sastavu kamenca, a pomodu SEM i AFM analiza interpretirane su morfološke značajke uzoraka. SEM rezultati pokazuju razliku u komponentama određenog kamenca, habitus kristalnih faza, te njihove strukture i teksture, dok su AFM metodom promatrane površinske jezgre kristalizacije i tragovi rasta kristala. Istraživani uzorci se razlikuju po mineralnom sastavu, te je analizama utvrđena prisutnost kalcijskih oksalata (whewellit, weddellit), fosfata (brushit, hidroksilapatit, struvit), urata i cistina.

Abstract (english)

Seven samples of renal calculi were analysed, which have been collected from patients in area of Zagreb and Osijek. Methods used for their analysis include X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). XRD results show the chemical composition of individual sample, and microscopic methods (SEM, AFM) are used to observe their morfological features. With SEM images individual compounds are observed and explained, including crystal habit, texture and structure of calculus. AFM images indicate different characteristics of crystal growth and the presence of surface nuclei. The composition of every sample is different. Mineral compounds in the samples include calcium oxalate (whewellite, weddellite), phosphate (brushite, hidroxiapatite, struvite), urate and cistine.

• Keywords: AFM; bubrežni kamenci; fosfati; mikroskopija; oksalati; SEM; XRD
• Keywords (english): AFM; microscopy; oxalate; phosphate; renal calculi; SEM; XRD
• Language: croatian
• URN:NBN: urn:nbn:hr:217:503848
• Study programme: Title: Graduate university study of Environmental Geology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra geologije (magistar/magistra geologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-02-09 11:45:06