Molekularno-filogenetička i filogeografska analiza vrste Ancylus fluviatilis O.F. Müller, 1774 (Gastropoda: Planorbidae) u Hrvatskoj

master's thesis

• Title (english): Molecular phylogenetic and phylogeographic analysis of Ancylus fluviatilis O. F. Müller, 1774 (Gastropoda: Planorbidae) in Croatia
• Author: Kristina Kocijan
• Mentor: Martina Podnar Lešić (mentor)
• Mentor: Jasna Lajtner (mentor)
• Committee member: Jasna Lajtner (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-11-09, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ancylus fluviatilis O. F. Müller, 1774 slatkovodni je puž iz porodice Planorbidae široko rasprostranjen u Hrvatskoj. A. fluviatilis najvjerojatnije predstavlja kompleks četiri genetski i reproduktivno izolirane kriptične vrste koje zasad još nisu formalno opisane. Cilj ovog istraživanja bio je, na temelju analize dva genska biljega, mitohondrijskih gena za COI i 16S rRNA, razotkriti koje su kriptične vrste A. fluviatilis kompleksa prisutne u Hrvatskoj i kakvo je njihovo rasprostranjenje u našim vodotocima te utvrditi molekularno-filogenetske odnose i genetske udaljenosti između populacija kao i filogeografski uzorak kompleksa na našem području. Utvrđeno je da Hrvatsku nastanjuju bar tri vrste A. fluviatilis kompleksa, široko rasprostranjen Ancylus sp. B, te lokalno prisutan Ancylus sp. C i A. fluviatilis sensu stricto čije rasprostranjenje je u skladu s općom filogeografskom slikom kompleksa. Filogeografski uzorak Ancylus sp. B u Hrvatskoj uglavnom se ne može objasniti prirodnim rasprostranjenjem i geografskim barijerama toku gena, nego pasivnim transportom i klimatskim značajkama područja koje nastanjuju.

Abstract (english)

Ancylus fluviatilis O. F. Müller, 1774 is a freshwater snail from family Planorbidae widespread in Croatia. A. fluviatilis most likely represents a complex of four genetically and reproductively isolated cryptic species that are not currently formally described. The aim of this study was, based on the analysis of two genetic markers, mitochondrial genes COI and 16S rRNA, to determine which cryptic species of A. fluviatilis complex are present in Croatia, their distribution, molecular phylogenetic relationships and genetic distance between populations as well as phylogenetic pattern that complex exhibits in our area. It was found that Croatia is inhabited with at least three species of A. fluviatilis complex, the widespread Ancylus sp. B, and locally present Ancylus sp. C and A. fluviatilis sensu stricto whose distribution is in accordance with the general phylogeographic picture of the complex. Observed phylogenetic pattern of Ancylus sp. B in Croatia mostly can not be explained by natural distribution and geographical barriers to gene flow, but rather to passive transport and climatic features of the area they inhabite.

• Keywords: kriptične vrste; slatkovodni puževi; filogenija; filogeografija; COI i 16S rRNA
• Keywords (english): cryptic species; freshwater snails; phylogeny; phylogeography; COI i 16S rRNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:265112
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-01-25 14:10:27