Nativno neuređeni proteini

undergraduate thesis

• Title (english): Intrinsically disordered proteins
• Author: Ingrid Tomljanović
• Mentor: Dubravko Pavoković (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nativno neuređeni proteini i proteinske regije (​engl. intrinsically disordered proteins/regions, IDP/IDR) pripadaju nedavno priznatoj skupini proteina koji su biološki aktivni usprkos nedostatku jasno definirane trodimenzionalne strukture. Za razliku od globularnih proteina, IDP u nativnom stanju ne posjeduju jedinstvenu stabilnu strukturu, već fluktuirajući skup strukturnih konformacija pogodan u staničnoj signalizaciji, regulaciji, šaperonskim aktivnostima, patogenezi bolesti i drugim raznovrsnim funckijama. Cilj ovog seminara bio je opisati osnovne značajke IDP te najvažnije eksperimentalne i bioinformatičke metode istraživanja IDP. Nadalje, rad obuhvaća podjelu IDP prema mehanizmu djelovanja i staničnoj ulozi te kratki osvrt na IDP u biljkama pri normalnim i stresnim uvjetima. U zaključku, područje IDP svjedočilo je strelovitom rastu u protekla dva desetljeća te se na temelju brojnosti i rasprostranjenosti ovih proteina predviđa velik potencijal za buduća istraživanja unutar ovog područja.

Abstract (english)

Intrinsically disordered proteins and protein regions (IDP/IDR) are a recently recognized group of proteins which are biologically active despite their inherent lack of a well-defined three-dimensional structure. Unlike globular proteins, IDPs lack a unique and stable 3D structure in their native state and exist as fluctuating ensembles of conformations which are well-suited for cellular signaling, regulation, chaperone activity, disease pathogenesis and other various functions. The aim of this Bachelor’s thesis is to describe the main characteristics of IDPs and the most important experimental and bioinformatic methods used in IDP research. Moreover, the thesis includes a classification of IDPs according to functional mechanism and cellular role, as well as a brief section on IDPs in plants in normal and stressful conditions. In conclusion, the IDP field has witnessed rapid growth in the past two decades and, based on the abundance and wide spread of these proteins, there is significant potential for future research within this field.

• Keywords: nativno neuređeni proteini
• Keywords (english): intrinsically disordered proteins
• Language: croatian
• URN:NBN: urn:nbn:hr:217:560806
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-04 10:38:47