Bioaktivni potencijal samoniklih jestivih gljiva Hrvatske

master's thesis

• Title (english): Bioactive potential of wild edible mushrooms of Croatia
• Author: Vedran Bahun
• Mentor: Armin Mešić (mentor)
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Ivan Čanjevac (član povjerenstva)
• Committee member: Blanka Cvetko Tešović (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-12-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kako bi se ustanovilo postoji li u plodištima 17 samoniklih jestivih vrsta gljiva iz odjeljka Basidiomycota sakupljenih u Hrvatskoj bioaktivni potencijal, u njihovim ekstraktima je analiziran sadržaj ukupnih topivih proteina, ukupnih polifenola, fenolnih kiselina, triterpenoida i alkaloida te antioksidacijski kapacitet (2,2-difenil-1-pikrilhidrazil (DPPH) test i test redukcije željeza/antioksidacijske snage (FRAP)). Sadržaj ukupnih topivih proteina u plodištima gljiva kretao se u rasponu 2,959–17,267 mg ekvivalenta goveđeg serumskog albumina (BSA)/g suhe težine (s.t.). Također, zabilježene su količine ukupnih polifenola u rasponu 1,492–26,374 mg ekvivalenta galne kiseline (GAE)/g s.t., fenolnih kiselina 0,733–6,336 mg ekvivalenta kavene kiseline (CAE)/g s.t., triterpenoida 16,371–61,184 mg ekvivalenta ursolinske kiseline (UAE)/g s.t. te alkaloida 33,376–116,710 mg ekvivalenta boldina (BE)/g s.t. Antioksidacijska aktivnost bila je najveća u plodištu vrste Agaricus sylvicola, na temelju rezultata dobivenih DPPH (199,698 μmol ekvivalenta Troloxa (TE)/g s.t.) i FRAP (365,773 μmol Fe2+/g s.t.). Visok sadržaj ukupnih polifenola i najviša antioksidacijska aktivnost izmjerena u plodištu vrste Agaricus sylvicola ukazuju na to da polifenoli izrazito doprinose antioksidacijskoj aktivnosti plodišta gljiva. Analizom rezultata ustanovljeno je da ispitivane vrste gljiva u plodištima sadrže različite skupine specijaliziranih metabolita što ukazuje na njihov značajan bioaktivni potencijal.

Abstract (english)

In order to determine if there is a bioactive potential in the fruitbodies of 17 wild edible mushroom species from phylum Basidiomycota collected in Croatia, the contents of total soluble proteins, total polyphenols, phenolic acids, triterpenoids and alkaloids, and antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays) were analyzed. Content of total soluble proteins in all fruitbodies ranged between 2,959 and 17,267 mg bovine serum albumin equivalent (BSA)/g of dry weight (dw). Furthermore, total polyphenols were measured in range 1,492–26,374 mg gallic acid equivalent (GAE)/g dw, phenolic acids 0,733–6,336 mg caffeic acid equivalent (CAE)/g dw, triterpenoids 16,371–61,184 mg ursolic acid equivalent (UAE)/g dw and alkaloids 33,376–116,710 mg boldine equivalent (BE)/g dw. Antioxidant activity was the highest in the fruitbody of Agaricus sylvicola, based on the results by DPPH (199,698 μmol Trolox equivalent (TE)/g dw) and FRAP (365,773 μmol Fe2+/g dw) method. High content of total polyphenols and the highest antioxidant activity measured in the fruitbody of Agaricus sylvicola indicate that polyphenols significantly contribute to the antioxidant activity of fungal fruitbodies. Based on the analysis of results, it was found that the studied species contain different groups of specialized metabolites which indicate their significant bioactive potential.

• Keywords: Fungi; Basidiomycota; plodišta; specijalizirani metaboliti; proteini; antioksidacijska aktivnost
• Keywords (english): Fungi; Basidiomycota; fruitbodies; specialized metabolites; proteins; antioxidative activity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:872087
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-31 09:21:25