Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom

master's thesis

• Title (english): Determination of thallium(I) acetate genotoxicity in common duckweed Lemna minor L. by comet assay
• Author: Petar Brnjilović
• Mentor: Mirjana Pavlica (mentor)
• Committee member: Mirjana Pavlica (predsjednik povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Koncentracija teškog metala talija u okolišu kontinuirano raste, a kako ga biljke mogu apsorbirati tako talij putem hranidbenih lanaca postaje dostupan životinjama i čovjeku. Cilj ovog istraživanja bio je pomoću komet-testa kao nespecifičnog biomarkera genotoksičnosti odrediti genotoksičan učinak talijeva(I) acetata u vodenoj leći Lemna minor L. Jezgre stanica vodene leće su prvo u uvjetima in vitro tretirane vodikovim peroksidom kao modelnim genotoksikantom čime je potvrđena osjetljivost komet-testa za utvrđivanje direktne genotoksičnosti na vodenoj leći. Vodena leća je sedam dana izlagana u uvjetima in vivo različitim koncentracijama talijeva(I) acetata. Rezultati komet-testa pokazali su blagi genotoksični učinak dvaju najviših koncentracija talija. Kako bi utvrdili djeluje li talij direktno ili indirektno na molekulu DNA izveden je i acelularni komet-test u uvjetima in vitro na jezgrama vodene leće. Rezultati dobiveni celularnim i acelularnim komet-testom ukazuju na indirektno oštećenje DNA uzrokovano talijevim(I) acetatom.

Abstract (english)

Concentration of the heavy metal thallium in the environment has been continuously increasing. Since thallium can be absorbed by plants it becomes available for the higher organisms including men through food chains. The main goal of this work was to determine genotoxicity of thallium(I) acetate by comet assay as a non-specific genotoxicity biomarker in common duckweed Lemna minor L. Duckweed cell nuclei in in vitro conditions were treated with hydrogen peroxide as a model genotoxicant in order to verify the comet assay sensitivity. Results are showing direct genotoxic effect. Common duckweed plants were in vivo exposed to different thallium(I) acetate concentrations for seven days. The results of comet assay showed a mild genotoxicity of two highest concentrations of thallium. In order to determine whether thallium directly or indirectly affect DNA an acellular comet assay was performed in vitro on common duckweed nuclei. Results following both cellular and acellular comet assays implies indirect DNA damage caused by thallium(I) acetate.

• Keywords: Lemna minor L.; talijev(I) acetat; komet-test; vodikov peroksid; genotoksiĉnost
• Keywords (english): Lemna minor L.; thallium(I) acetate; comet assay; hydrogen peroxide; genotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:140032
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-07 09:21:27