Važnost proteinskih domena MATH i BTB u određivanju polarnosti biljnih stanica

master's thesis

• Title (english): Importance of protein domains MATH and BTB in establishment of the plant cell polarity
• Author: Ivana Šumanovac
• Mentor: Dunja Leljak-Levanić (mentor)
• Committee member: Dunja Leljak-Levanić (predsjednik povjerenstva)
• Committee member: Željka Vidaković Cifrek (član povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nastanak različitih tipova stanica iz zigote višestaničnih organizama temelji se na postojanju unutarstanične polarnosti i asimetrične diobe. U zigotama i ranim stupnjevima razvoja embrija pšenice nađen je protein TaMAB2. U ovom radu je ispitan utjecaj dviju domena proteina TaMAB2, MATH i BTB, na uspostavljanje njegovog polarnog rasporeda i na asimetričnu diobu. Radi praćenja lokalizacije je korištena tehnika obilježavanja proteina GFP-om i postupak transformacije Biolistikom. Delecije su načinjene PCR-om. Delecija svake domene je utjecala na karakterističnu polarnu lokalizaciju proteina TaMAB2 mijenjajući njegov polaran raspored u stanici. Pri tome je delecija MATH domene u svim mikroskopiranim BMS stanicama uzrokovala promjenu afiniteta nakupljanja i polarne lokalizacije uz jezgrinu ovojnicu. Delecija BTB domene je uzrokovala promjenu polarne lokalizacije proteina TaMAB2 u 74% mikroskopiranih BMS stanica. Mikroskopiranje stanica epiderme luka je pokazalo slične rezultate. Ipak, još uvijek se ne zna točna uloga TaMAB2 u uspostavljanju stanične polarnosti i asimetrične diobe zigote.

Abstract (english)

Development of different cell types from the zygote of multicellular oraganism is based on the establishment of the the cell polarity and asymmetric cell division. Protein TaMAB2 was found in the zygote and early embryonic stages of wheat. In this theisis influence of the protein domains of TaMAB2, MATH and BTB, on it's charactristic polar localisation on nuclear envelope was analysed. For this purpose GFP tagging of proteins was used for plant cell transformation. Deletions were made by using PCR. It was found that deletion of each domain influence the protein localisation by disturbing its polar distribution. While deletion of the MATH domain disturbed polar distribution in all BMS cells, deletion of the BTB domain changed polar localisation of TaMAB2 in 74% of BMS cells. Analysis of epidermal onion cells showed similar results. Precise role of TaMAB2 in establishment of the plant cell polarity and asymmetric cell division is still unknown.

• Keywords: stanična polarnost; asimetrična dioba zigote; protein TaMAB2; proteinske domene MATH i BTB
• Keywords (english): plant cell polarity; asimmetic cell division; zygote; protein TaMAB2; protein domains MATH and BTB
• Language: croatian
• URN:NBN: urn:nbn:hr:217:792284
• Study programme: Title: Biology; specializations in: Molecular biology; Course: Molecular biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-21 11:27:36