Inhibicija virusa TBSV ekstraktima kalusa vrste Centaurea rupestris L.

master's thesis

• Title (english): Inhibition of TBSV infection using extracts of Centaurea rupestris L. calli
• Author: Marijana Juren
• Mentor: Mirna Ćurković-Perica (mentor)
• Mentor: Gordana Rusak (sumentor)
• Committee member: Draginja Mrvoš-Sermek (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ekstraktima cvjetova i listova vrste Centaurea rupestris L. već je ranije dokazano prisustvo flavonoida. Ekstrakti cvjetova i listova kao i flavonoid kvercetagetin 3'-metileter-7-O-β-D-glukopiranozid izoliran iz cvjetova vrste C. rupestris već su pokazali protuvirusni učinak na virus grmolike kržljavosti rajčice,Tomato bushy stunt virus, (TBSV). U ovom je radu spektrofotometrijski određen sadržaj flavonoida u tkivima vrste C. rupestris uzgojenim in vitro na hranidbenoj podlozi s različitim koncentracijama nutrijenata i biljnih regulatora rasta. Sadržaj flavonoida izmjeren u tkivima bio je manji od sadržaja flavonoida u cvjetovima i listovima biljaka sa prirodnog staništa. Ekstrakti tkiva uzgojenih in vitro imali su protuvirusni učinak na TBSV, ali je taj učinak bio slabiji od učinka ekstrakta cvijeta.

Abstract (english)

In extracts of Centaurea rupestris L. flowers and leaves, the presence of flavonoids has already been proven. Those extracts, along with the flavonoid kvercetagetin 3'-methyl-7-O-β-D-glucopyranosid, which was isolated from flowers of C. rupestris, have demonstrated the antiviral activity against Tomato bushy stunt virus (TBSV). In this paper the content of flavonoids was spectrophotometrically determined in tissues of C. rupestris propagated in vitro, on medium supplemented with different concentrations of nutrients and plant growth regulators. The content of flavonoids measured in tissues grown in vitro was lower than the flavonoid content in flowers and leaves of naturally-growing plants. The antiviral activity of extracts from in vitro grown tissues against TBSV was confirmed, but it was weaker than the antiviral activity of flower extract.

• Keywords: endem; flavonoidi; protuvirusna aktivnost
• Keywords (english): antiviral activity; endem; flavonoids
• Language: croatian
• URN:NBN: urn:nbn:hr:217:745525
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-06 10:43:24