Škrob kao indikator zametanja mikrogomolja krumpira (Solanum tuberosum L.)

master's thesis

• Title (english): Atarch as as indicator of microtuberization in the potato (Solanum tuberosum L.)
• Author: Jasmina Šilić
• Mentor: Branka Pevalek-Kozlina (mentor)
• Committee member: Branka Pevalek-Kozlina (član povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Razvitak gomolja krumpira je složeni razvojni proces koji uključuje promjenu razine regulatora rasta, sintezu specifičnih proteina i odlaganje škroba. Formiranje gomolja kontroliraju uvjeti kratkog dana i niske temperature. U uvjetima in vitro gomolji se mogu razviti iz pazušnih pupova na stabljici krumpira. U sklopu ovog diplomskog rada istraživano je odlaganja škroba u stanicama bočnih pupova izdanka krumpira (Solanum tuberosum L. cv. ΄Bintje΄) kao prvog znaka razvoja mikrogomolja. Biljke korištene u pokusima razmnožavane su nodijskim segmentima na podlozi MS uz dodatak 2% saharoze i 0,8% agara u razmacima od 4 tjedna. Segmenti stabljike presađeni su na podlogu MS modificiranu za razvitak gomolja koja je sadržavala 8% saharoze, 0,8% agara i 22 μM BA. Kulture su inkubirane na 20 °C u uvjetima kratkog dana (fotoperiod 12 sati). Za histološke analize pupovi su uzimani svaki dan tijekom 14 dana, fiksirani i bojani Lugolovom otopinom (IKI2) prije uklapanja u glicerol. Prvi znak odlaganja škroba uočen je treći dan pokusa u bazalnom dijelu bočnih pupova na podlozi za tuberizaciju. U eksplantatima uzgajanim u jednakim uvjetima, ali na podlozi bez BA odlaganje škroba nije uočeno niti nakon 14 dana. Četiri dana nakon prve pojave škroba bočni su pupovi zadebljali i tijekom sljedećih dana iz njih su se razvili mikrogomolji. Ovi rezultati potvrđuju važnost visoke koncentracije saharoze i prisutnosti BA u hranjivoj podlozi kao i duljine fotoperioda i temperature u procesu tuberizacije krumpira.

Abstract (english)

Formation of potato tubers is a complex developmental system which includes important biological processes such as growth regulator level changes, synthesis of specific proteins and starch deposition. Tuber formation is controlled by short day and low temperature conditions. Axillary buds of potato stem can also produce tubers in in vitro conditions. Considering these facts the first sign of starch deposition during early development of microtubers in axillary buds of potato (Solanum tuberosum L. cv. ΄Bintje΄) has been investigated. Potato plantlets used in the experiment were microcloned by nodal segments on MS medium with 2% sucrose and 0.8% agar in 4 week intervals. Stem segments were transferred on mod. MS medium for tuberization supplemented with 8% sucrose, 0.8% agar and 22 μM BA. Cultures were maintained at 20 ºC under short day conditions (12 hr photoperiod). For hystological analysis buds were taken every day for 14 days, fixed and stained with Lugol solution (IKI2) before embedding in glycerole. The first sign of starch deposition was noticed on day three in the basal part of axillary buds on tuber stimulative medium. In explants grown under same conditions but on medium without BA starch deposition was not noticed even after 14 days in culture. Four days after the occurence of the first starch deposition axillary buds were thickened and formed oval microtubers in next days. This confirms the importance of high sucrose concentrations and BA presence in nutrient medium as well as short photoperiod and temperature in the tuberization process of potatoes.

• Keywords: Solanum tuberosum; mikrogomolj; škrob
• Keywords (english): Solanum tuberosum; microtuber; starch
• Language: croatian
• URN:NBN: urn:nbn:hr:217:241880
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-25 12:41:08