Izraženost biljega Iba1, CD68 i HLA-DR aktiviranih mikroglija stanica u hipokampalnoj formaciji osoba oboljelih od Alzheimerove bolesti

master's thesis

• Title (english): Expression of activated microglia cells markers Iba1, CD68 and HLA-DR in the hippocampal formation of patients with Alzheimer's disease
• Author: Ena Španić
• Mentor: Goran Šimić (mentor)
• Mentor: Dubravka Hranilović (sumentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Brojna istraživanja govore o štetnom utjecaju upalnih procesa i mikroglija stanica na razvoj Alzheimerove bolesti (AB). Stoga se u okviru diplomskog rada imunohistokemijskom metodom analizirao izražaj biljega prisutstva i aktivacije mikroglija stanica Iba1, CD68 i HLA-DR u uzorcima hipokampalne formacije zahvaćenim Alzheimerovom bolešću i kontrolnim uzorcima. Ciljevi istraživanja bili su ispitati izraženost biljega u područjima hipokampalne formacije (sloj zrnatih stanica nazubljene vijuge, hilus, CA3/2, CA1, subikul, bijela tvar), utvrditi postoji li pozitivna korelacija izražaja biljega s duljinom trajanja AB i usporediti izražaj biljega aktivirane mikroglije, CD68 i HLA-DR s brojem amiloidnih plakova i neurofibrilarnih snopića u područjima hipokampalne formacije. Istraživanje je pokazalo da se izražaj biljega mikroglija stanica razlikuje u mozgu osoba oboljelih od AB i kontrolnih ispitanika. Pronađena je pozitivna korelacija izražaja biljega aktivirane mikroglije, CD68 i HLA-DR s pojavom AB i njihov povećani izražaj u područjima hipokampalne formacije koja su najviše zahvaćena patologijom AB, te negativna korelacija izražaja Iba1 s AB. Dobiveni rezultati podupiru hipoteze o ulozi upale u neurodegenerativnim bolestima i štetnom utjecaju mikroglija stanica u AB.

Abstract (english)

Numerous studies indicate the harmful effect of inflammatory processes and microglia cells in Alzheimer's disease (AD). Therefore, within the graduate thesis, we analyzed expression of microglia cells markers Iba1, CD68 and HLA-DR in the hippocampal formation of patients with Alzheimer's disease and control samples. The main goals were to determine expression of markers in the different areas of hippocampal formation (granular cell layer of dentate gyrus, hilus, CA2/3, CA1, subiculum and white matter), to determine whether there is a positive correlation between expression of the markers and the duration of AD and to compare the expression of activated microglia cells markers, CD68 and HLA-DR with numbers of amyloid plaques and neurofibrillary tangles in different areas of hippocampal formation. The research has shown that the expression of microglia markers differs in the AD brains and controls. There was a positive correlation between expression of activated microglia cells markers CD68 and HLA-DR with the AD and their higher expression in the areas of hippocampal formation most affected by AD pathology and the negative correlation of expression of Iba1 with the AD. These results support the inflammatory hypothesis of neurodegenerative diseases and the harmful effect of the microglia cells in the AD.

• Keywords: Alzheimerova bolest; upala; mikroglija stanice; imunohistokemija
• Keywords (english): Alzheimer's disease; inflammation; microglia cells; imunohistochemical method
• Language: croatian
• URN:NBN: urn:nbn:hr:217:362315
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-08-28 10:47:47