Ekspresija proteina p53 i p73 u uvjetima oksidacijskog stresa izazvanog vodikovim peroksidom u stanicama SH-SY5Y

master's thesis

• Title (english): Expression of p53 and p73 protein in hydrogen peroxide-induced oxidative stress in SH-SY5Y cells.
• Author: Snježana Dunđer
• Mentor: Maja Jazvinšćak Jembrek (mentor)
• Committee member: Morana Dulić (predsjednik povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Aleksandra Maršavelski (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-10-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Mnogi pristupi u liječenju tumora temelje se na izazivanju oksidacijskog stresa i posljedično apoptoze primjenom ionizirajućeg zračenja ili kemoterapeutika. Cilj ovog istraživanja je bio ispitati utjecaj oksidacijskog stresa, izazvanog primjenom vodikovog peroksida (H2O2), na ekspresiju tumor supresorskih proteina p53 i p73, koji su uključeni u pokretanje apoptoze. Istraživanje je provedeno u stanicama neuroblastoma, stanična linija SH-SY5Y. Utvrdili smo da u ovisnosti o primijenjenoj koncentraciji, H2O2 smanjuje preživljenje stanica, unutarstaničnu koncentraciju glutationa i ATP-a, te da povećava nakupljanje reaktivnih kisikovih vrsta (ROS). Metodom western blot utvrdili smo porast u ekspresiji ukupnog proteina p53 te njegove izoforme p53α, dok je ekspresija izoformi p53β i Δ40p53β specifično smanjena. Također, nakon izlaganja H2O2 došlo je do jake indukcije ekspresije proteina p73, posebice izoforme TAp73α. Dobiveni rezultati upućuju na značajan doprinos pojačane ekspresije proteina p53 i p73 u smanjenju preživljenje stanica SH-SY5Y u uvjetima oksidacijskog stresa.

Abstract (english)

Many approaches in tumor therapy are based on oxidative stress induction and triggering apoptosis by using ionizing radiation or chemotherapeutics. The aim of the study was to examine the effect of hydrogen peroxide (H2O2)-induced oxidative stress on the expression of tumor suppressor proteins p53 and p73 that are involved in the initiation of apoptosis. The study was performed in the neuroblastoma cell line SH-SY5Y. In a concentration-dependent manner H2O2 decreased cell survival, intracellular glutathione and ATP levels, and increased accumulation of reactive oxygen species (ROS). Western blot method revealed increased expression of the total p53 protein and its isoform p53α, while the expression of isoforms p53β and Δ40p53β was specifically reduced. Furthermore, after H2O2 exposure, strong induction of p73 expression, particularly the TAp73α isoform, was found. The obtained results suggest that increased expression of p53 and p73 proteins greatly contribute to reduced survival of SH-SY5Y cells under oxidative stress conditions.

• Keywords: vodikov peroksid; oksidacijski stres; tumori; proteini
• Keywords (english): hydrogen peroxide; oxidative stress; proteins; tumor
• Language: croatian
• URN:NBN: urn:nbn:hr:217:607315
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-10-22
• Created on: 2018-11-26 14:05:03