Određivanje specijaliziranih metabolita u raštici (Brassica oleracea var. acephala)

master's thesis

• Title (english): Evaluation of specialized metabolites in kale (Brassica oleracea var. acephala)
• Author: Petra Ivanišević
• Mentor: Dunja Šamec (mentor)
• Mentor: Ivana Šola (mentor)
• Committee member: Ivana Šola (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-11-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Raštika (Brassica oleracea L. var. acephala) je biljka iz porodice kupusnjača bogata
specijaliziranim metabolitima s pozitivnim utjecajima na ljudsko zdravlje. U Hrvatskoj nije
razvijena komercijalna proizvodnja sjemena te postoji znatna intra- i interpopulacijska
varijabilnost. U ovom radu hidroponski je uzgojena raštika čije je sjeme bilo podrijetlom iz
Mostara, Vrgorca, Gornje Brele, Pule, Pelješca i od komercijalnog proizvođača Bejo te su
ispitani klijavost, morfološke karakteristike, sadržaj specijaliziranih metabolita i
antioksidacijska aktivnost kako bi se odredilo potencijalne razlike između populacija.
Najbolju klijavost i prinos pokazale su populacije iz Mostara i Vrgorca, a najmanju ona s
Pelješca. Sadržaj ukupnih polifenola, fenolnih kiselina, glukozinolata i karotenoida nije se
statistički značajno razlikovao u uzorcima uzgojenim od različitih proizvođača sjemena.
Sadržaj ukupnih flavonoida bio je statistički najviši kod raštike od proizvođača sjemena s
Pelješca dok je najvišu antioksidacijsku aktivnost pokazala raštika uzgojena iz sjemena iz
Vrgorca. Sve istražene populacije sadrže značajne količine specijaliziranih metabolita na koje
podrijetlo sjemena nema značajan utjecaj (osim na sadržaj ukupnih flavonoida i
antioksidacijsku aktivnost).

Abstract (english)

Kale (Brassica oleracea L. var. acephala) is a cruciferous plant rich in specialized
metabolites with positive effects on human health. There is no commercially available kale
seeds in Croatia what resultes with the considerable intra- and interpopular variability. In the
present work, we grown soilless plants from the seeds originated from Mostar, Vrgorac,
Gornja Brela, Pula, Pelješac and from only commercial producer Bejo in order to determinate
the influence of seed origin on germination percentige, morphological characteristics,
specialized metabolites level and antioxidant activity. The best germination and yield had
populations from Mostar and Vrgorac while the smallest ones has Peljesac. The content of
total polyphenols, phenolic acids, glucosinolates and carotenoids did not differ significantly in
the samples grown from different seed producers. The content of total flavonoids was the
highest in kale from Peljesac while the highest antioxidant activity was shown by kale from
Vrgorac seeds. All evaluated populations contain significant amounts of specialized
metabolites with health benefites and seed origin mostly did not have significant effect on
metabolites level, except slightly on the total flavonoid content and antioxidant activity.

• Keywords: Brassicaceae; hidroponski uzgoj; fitokemikalije; antioksidacijska aktivnost
• Keywords (english): Brassicaceae; hydroponic breeding; phytochemicals; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:735423
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-11-28
• Created on: 2019-03-28 11:37:24