Preživljavanje okolišnih izolata bakterije Acinetobacter baumannii u vodenim medijima

master's thesis

• Title (english): Survival of environmental isolates of bacterium Acinetobacter baumannii in water media
• Author: Iva Tomašin
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Marko Miliša (član povjerenstva)
• Committee member: Ivana Ivančić Baće (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bakterija Acinetobacter baumannii oportunistički je patogen i vodeći uzročnik bolničkih infekcija na svijetu. Nedavni nalazi A. baumannii u bolničkim vodama, urbanoj kanalizaciji i rijekama ukazuju na prisustvo ove bakterije u prirodnom okolišu, što predstavlja javnozdravstveni rizik. Cilj ovog rada bio je proučiti sposobnosti preživljavanja okolišnih izolata bakterije A. baumannii u različitim vodenim medijima na 4, 22 i 44 °C kroz 50 dana praćenja kako bi se moglo predvidjeti njeno ponašanje u okolišu. Najbolju sposobnost preživljavanja bakterija pokazuje na sobnoj temperaturi (22 °C) u destiliranoj vodi obogaćenoj hranjivima. Najlošije preživljavanje od 57 % zabilježeno je u izvorskoj vodi na 44 °C. Na 4 °C u izvorskoj vodi i hranjivima bogatim vodenim medijima (destilirana voda i izvorska voda) bakterija ne pokazuje mogućnost razmnožavanja. Istraživanjem je zaključeno da bakterija A. baumannii ima sposobnost preživljavanja svih 50 dana u svim testiranim uvjetima.

Abstract (english)

Acinetobacter baumannii is considered to be an opportunistic pathogen and one of the leading causes of hospital infections in the world. Recent findings of A. baumannii in hospital wastewaters, urban sewage and natural recipients shows presence of this bacteria in natural environment, which presents a potential threat to public health. The aim of this thesis is to determine the time of survival of environmental isolates of A. baumannii in different water media at 4, 22 and 44 °C for up to 50 days of monitoring in order to predict its environmental behaviour. The highest survival rate was at room temperature (22 °C) in distilled water with nutrient broth. The worst survival (57 %) was observed in spring water at 44 °C. At 4 °C in spring water and nutrient enriched waters (spring and distilled water), the bacterium did not show the possibility of multiplication. The results show that A. baumannii is able to survive during 50 days of monitoring in all tested conditions.

• Keywords: bakterija; patogen; ekološki čimbenici; temperatura; voda
• Keywords (english): bacteria; pathogen; ecological factors; temperature; water
• Language: croatian
• URN:NBN: urn:nbn:hr:217:605130
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-10 08:14:27