Utjecaj satelitne DNA na regulaciju gena u brašnara Tribolium castaneum (Herbst 1797)

master's thesis

• Title (english): Study of satellite DNA-mediated gene regulation in red flour beetle Tribolium castaneum (Herbst, 1797)
• Author: Ivana Akrap
• Mentor: Đurđica Ugarković (mentor)
• Committee member: Vlatka Zoldoš (predsjednik povjerenstva)
• Committee member: Đurđica Ugarković (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Dijana Škorić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-02-11, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Satelitne DNA predstavljaju uzastopno ponavljajuće sekvence koje čine značajan dio genoma eukariotskih organizama. Glavni su građevni elementi pericentromernog i centromernog hetrokromatina. Njihove strukturne odlike, očuvani motivi i transkripcijska aktivnost upućuju također na njihovu ulogu u regulaciji ekspresije gena. TCAST predstavlja glavnu satelitnu DNA u brašnara Tribolium castaneum. Raspršeni oblik TCAST-a je pronađen u blizini velikog broja (101) unikatnog gena. U ovome radu otkriven je polimorfizam raspršenog satelita TCAST među sojevima brašnara T. castaneum i kao mogući mehanizam raspršenja satelita predložena je mjesno-specifična rekombinacija. Dobiveni rezultati također upućuju na moguću ulogu raspršenog satelita TCAST u regulaciji gena koji se nalaze u njegovoj blizini, u uvjetima temperaturnog stresa.

Abstract (english)

Satellite DNAs are tandemly repeated sequences that constitute a considerable part of the genomic DNA of many eukaryotic organisms. They represent a major building element of pericentromeric and centromeric heterochromatin. The existence of conserved motifs and structural properties and their transcriptional activity suggest that, in addition to participating in centromere and heterochromatin formation, satellite DNAs might also act as cis-regulatory elements of gene expression. TCAST represents major satellite DNA in the red flour beetle Tribolium castaneum. Dispersed form of TCAST satellite was associated with 101 proteincoding genes. This study reveals polymorphism of dispersed TCAST satellite-like elements among strains of T. castaneum and gives a strong indication for the role of site specific recombination as a mechanism of dispersion of repetitive elements. The results of this study also suggest the role of dispersed TCAST satellite-like elements in regulation of genes located in their vicinity, under specific heat stress conditions.

• Keywords: cis- regulatorni elementi; polimorfizam satelitnih sekvenci; satelit TCAST
• Keywords (english): cis-regulatory elements; satellite polymorphism; TCAST satellite
• Language: croatian
• URN:NBN: urn:nbn:hr:217:628381
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 10:35:08