Uklanjanje fosfata iz otpadnih voda pomoću bakterije Acinetobacter junii imobilizirane na komercijalne materijale

master's thesis

• Title (english): Removal of phosphates from wastewater by bacteria Acinetobacter junii immobilised on commercial materials
• Author: Nikolina Bolf
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog rada bio je istražiti mogućnost uklanjanja fosfata iz otpadnih voda pomoću tri vrste komercijalnih materijala na koje su imobilizirane fosfat-akumulirajuće bakterije Acinetobacter junii. Bakterije na materijalima prvo su uzgojene u C-mediju (s niskom koncentracijom fosfata), a zatim prebačene u P-medij (s visokom koncentracijom fosfata). Praćeno je koji je od tri korištena materijala (ugljična vlakna, Culti-Spher S i piljevina) najpogodniji za imobilizaciju bakterija, prilagodljiv što se tiče uvjeta u kojima se nalazi te da uspješno uklanja fosfate iz otpadne vode. Broj imobiliziranih bakterija bio je najveći na materijalu Culti-Spher S (4,9×109 CFU g-1), no on nije pogodan za uklanjanje fosfata jer mu se u vodi narušila struktura i rastopio se. Velik broj imobiliziranih bakterija (1,9×109 CFU g-1) bio je na ugljičnim vlaknima i to je bio materijal na kojemu su bakterije učinkovito uklanjale fosfate iz otpadne vode. Na piljevini je bilo 4,3×108 CFU g-1 imobiliziranih bakterija, no voda s piljevinom je potamnila i došlo je do otpuštanja fosfata.

Abstract (english)

The aim of this study was to investigate the possibility of phosphate removal from wastewater using the three types of commercial materials onto which phosphate accumulating bacteria Acinetobacter junii were immobilized. Bacteria on the materials were first grown in C-medium (with low concentration of phosphate) and then transferred to the P-medium (with high concentration of phosphate). We followed which of the three used materials (carbon fiber, Culti-Sphere S and sawdust) were the most suitable for the immobilization of bacteria, customizable in the conditions in which they are located and were successful in P removal. The number of immobilized bacteria was the highest on the material Culti-Spher S (4,9×109 CFU g-1) but this material was not suitable for the removal of phosphate due to the collapse of the structure. Large number of bacteria was immobilized on the carbon fiber (1,9×109 CFU g-1) and it was material on which the bacteria removed phosphates effectively. The sawdust had 4,3×108 CFU g-1 of immobilized bacteria but water with sawdust turned dark and release of phosphate from the material occurred.

• Keywords: fosfat-akumulirajuće bakterije; pojačano biološko uklanjanje fosfata (EBPR); Acinetobacter junii; komercijalni materijali; fosfati
• Keywords (english): phosphate-accumulating bacteria; EBPR process; Acinetobacter junii; commercial materials; phosphates
• Language: croatian
• URN:NBN: urn:nbn:hr:217:812434
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 12:15:35