Učinak resveratrola i njegovih nanokristala na angiogenezu, oksidacijski stres i rast solidnog tumora

master's thesis

• Title (english): Effect of resveratrol and its nanocrystals on angiogenesis, oxidative stress and proliferation of solid tumor
• Author: Matea Tomašević
• Mentor: Nada Oršolić (mentor)
• Committee member: Jasna Lajtner (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-06-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog istraţivanja bio je istraţiti učinak resveratrola i njegovih nanokristala u dozi od 50 mg/kg na rast brzo rastućeg Ehrlichovog solidnog tumora (EAT) ovisnog o angiogenezi, oksidacijski stres i potencijalnu toksičnost nastalu nakupljanjem nanokristala u jetri i bubregu miševa te polarizaciju makrofaga. Tumor je izazvan injiciranjem 1 × 106 stanica EAT subkutano (sc) u bedro miševa soja Swiss albino. Obrada ţivotinja s EAT započeta je prvi dan pokusa intratumoralanim (it) injiciranjem resveratrola ili nanokristala resveratrola u dozi od 50 mg/kg svaki drugi dan tijekom 14 dana. Dobiveni rezultati pokazuju da it primjena resveratrola ili nanokristala resveratrola posjeduje izrazito anti-angiogenetsko i inhibicijsko djelovanje na rast EAT u solidnom obliku. Smanjena mikroţilna gustoća u tkivu jetre i bubrega potvrĎuje anti-angiogeni potencijal resveratrola in vivo. Moţemo zaključiti da resveratrol i njegovi nanokristali primijenjeni it uspješno inhibiraju rast i angiogenezu solidnog oblika Ehrlichovog ascitesnog tumora te zaštitno djeluju na jetru i bubreg kroz antioksidacijsku i protuupalnu aktivnost.

Abstract (english)

The aim of this study was to investigate the effect of resveratrol and its nanocrystals at concentration of 50 mg/kg on the growth of angiogenetically-dependent, fast-growing Ehrlich solid tumor (EAT), oxidative stress and potential toxicity caused by accumulation of nanocrystals in the liver and the kidney of mice and macrophage polarization. The tumor was induced by subcutaneous (sc) injection of 1 × 106 EAT cells into the thigh of Swiss albino mice. Treatment of animals with EAT started on day 1 of the experiment by intratumoral (it) injection of resveratrol or resveratrol nanocrystals at a dose of 50 mg/kg every other day for 14 days. The obtained results indicate that it administration of resveratrol or resveratrol nanocrystals has an anti-angiogenic and inhibitory effect on EAT growth in solid form. Reduced microvessel density in the liver and kidney tissue confirms the anti-angiogenic potential of resveratrol in vivo. Summarizing the results, we can conclude that resveratrol and its nanocrystals applied it successfully inhibit the growth and angiogenesis of a solid form of Ehrlich ascites tumor and have a protective effect on the liver and kidney through antioxidant and anti-inflammatory activity.

• Keywords: solidni tumor; resveratrol; nanokristali; angiogeneza; oksidacijski stres
• Keywords (english): solid tumor; resveratrol; nanocrystals; angiogenesis; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:130578
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-06-26
• Created on: 2020-07-09 10:42:59