Primjena mikročestica zeolita A u regulaciji glikemije dijabetičnih miševa soja NOD

master's thesis

• Title (english): Application of zeolite A micropartictes in the regulation of glycemia in diabetic NOD mice
• Author: Adrijana Perković
• Mentor: Marijana Popović Hadžija (mentor)
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Dubravko Pavoković (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-11-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Šećernu bolest karakterizira nemogućnost tijela da proizvodi i/ili pravilno upotrebljava hormon inzulin. Posljedica je povećana koncentracija glukoze u krvi. U našem radu koristili smo miševe NOD koji spontano razviju šećernu bolest tipa 1 i za regulaciju bolesti dobivaju inzulin. Unatoč inzulinu, koncentracija glukoze je viša od preporučene, i zato za bolju regulaciju bolesti treba iskoristiti još neki fiziološki proces. Stoga smo istražili mogućnost (barem djelomične) inhibicije apsorpcije glukoze. Koristili smo kalcijev oblik zeolita A. Svojstvo vezanja natrija u strukturu zeolita smo iskoristili budući se zna da je za ulazak glukoze u stanicu i dalje u krv, potreban natrij. Hipoteza je bila da ćemo obradom sa zeolitom smanjiti koncentraciju iona natrija u probavnom traktu. Direktan učinak zeolita na apsorpciju glukoze pratili smo mjereći glukozu nakon hranjenja i nakon testa tolerancije glukoze. Rezultati su u oba slučaja pokazali bolju niži nivo šećera u krvi kod dijabetičnih miševa koji su primali zeolit A. Snimanjem anesteziranih životinja microPET metodom pokazali smo da se radioaktivno obilježena glukoza, nakuplja u mokraćnom mjehuru u znatno većoj mjeri ako su miševi dobili sondu Ca zeolita. Rezultati ovog rada jasno pokazuju pozitivan učinak primjene Ca zeolita A u regulaciji glikemije kod miševa oboljelih od šećerne bolesti.

Abstract (english)

Diabetes is characterized by the inability of the body to produce and/or properly use the hormone insulin. The result is an increased concentration of glucose in the blood. We used NOD mice that spontaneously developed type 1 diabetes and received insulin for the regulation of the disease. Despite today’s therapy glucose level was higher than recommended, and therefore it was necessary to take advantage of some other physiological processes in order to regulate the disease more efficiently. We investigated the ability to inhibit glucose absorption by the treatment with calcium zeolite A. The property of sodium binding was used because it is known that sodium is required for the entry of glucose into the cells. The hypothesis was that we would reduce the concentration of sodium ions in the gastrointestinal tract with the help of the zeolite treatment. The direct effect of Ca zeolite at the absorption of glucose was monitored by measuring glucose concentration after eating
and OGTT. Results have shown better disease control in diabetic mice treated with zeolite. By recording anesthetized animals with the microPET method, we showed that radioactively labeled glucose accumulated to a greater extent in the bladder if the mice received the zeolite probe. The results of this study clearly demonstrate the positive effect of applying of micro particles Ca zeolite A on blood glucose levels in mice with diabetes.

• Keywords: Diabetes mellitus; Ca zeolit A; microPET; NOD miševi
• Keywords (english): Diabetes mellitus; Ca zeolite A; microPET; NOD mice
• Language: croatian
• URN:NBN: urn:nbn:hr:217:598969
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-26 11:07:51