Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2

master's thesis

• Title (english): The impact of polystyrene and silver nanoparticles on the viability and oxidative stress of Jurkat AND HepG2 cell lines
• Author: Ana Hubak
• Mentor: Ivan Pavičić (mentor)
• Mentor: Sandra Radić Brkanac (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Jurkat stanična linija je besmrtna stanična linija T-limfocita koja je izvorno dobivena iz periferne
krvi četrnaestogodišnjaka oboljelog od akutne limfoblastične leukemije, a HepG2 stanična linija
je epitelna stanična linija koja je dobivena iz jetre hepatocelularnog karcinoma
petnaestogodišnjeg dječaka. Obje stanične linije korištene su kao modelne stanične linije za
ispitivanje toksičnosti nanočestica polistirena i srebra koje su bile stabilizirane različitim
omotačima. Osim određivanja preživljavanja staničnih linija nakon tretmana s nanočesticama srebra
i polistirena, mjerili smo i razine biomarkera oksidativnog stresa primjenom fluorescentnih proba
diklorodihidroflourescein diacetata i monoklorobimana. Rezultati dobiveni metodom Live/Dead na
protočnom citometru pokazuju da se kombinirani tretman nanočestica na Jurkat staničnoj liniji
pokazao citotoksičnijim od pojedinačnih tretmana s istim koncentracijama nanočestica. Dobiveni
rezultati također pokazuju da je Jurkat stanična linija osjetljivija od HepG2 stanične linije. Nakon
tretmana nanočesticama srebra i polistirena mjeren je ukupan sadržaja glutationa (GSH) i
reaktivnih kisikovih vrsta u staničnim linijama Jurkat i HepG2 te nije dokazana pojava oksidativnog
stresa.

Abstract (english)

Jurkat cell lines are immortalized T-lymphocytes first derived from the peripheral blood of a child
suffering from leukemia. HepG2 cell line is an immortalized cell line of human liver carcinoma
cells, derived from the liver tissue of child suffering from hepatocellular carcinoma. In this
research, we used cell lines as a test organism to assess toxicity of polystyrene and silver
nanoparticles stabilized with different biomolecules. Besides determining the survival of cell lines
after the treatment with polystyrene and silver nanoparticles we, also measured levels of
oxidative stress biomarkers by using fluorescent probes dichloro-dihydro-fluorescein diacetate
and monochlorobimane. Results with Live/Dead flow cytometry tests demonstrated that
combined treatment of Jurkat cells with both PVPAgNP and PSNPs has proven to be more
cytotoxic than individual treatments with the same nanoparticle concentrations. Obtained
results also show that Jurkat cell line proved to be more sensitive than the HepG2 cell line.
Oxidative stress response in cells was detected by measuring total amount of reactive oxygen
species and glutathione. PVPAgNP and PSNP did not trigger oxidative in Jurkat and HepG2 cell
lines.

• Keywords: nanočestice srebra; polistirenske nanočestice; polistiren; vijabilnost; oksidativni stres; Jurkat; HepG2
• Keywords (english): silver nanoparticles; polystyrene nanoparticles; polystyrene; viability; oxidative stress; Jurkat; HepG2
• Language: croatian
• URN:NBN: urn:nbn:hr:217:045746
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-20 11:24:41