Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu

undergraduate thesis

• Title (english): CRISPR/Cas9 system in genetic engineering
• Author: Dora Lalić
• Mentor: Vlatka Zoldoš (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-08-31, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Promjene u nukleotidnoj sekvenci DNA, kao i epigenetičke promjene koje rezultiraju
perturbacijom kromatinske strukture, mogu dovesti do razvitka mnogih bolesti u ljudi i životinja.
Otkriće sustava CRISPR/Cas9 koji potječe od prokariotskih organizama u kojima ima ulogu
imunosnog sustava, omogućilo je determinaciju i istraživanje kodirajuće i nekodirajuće DNA,
aktivaciju ili represiju genske aktivnosti te uvođenje mjesno-specifičnih mutacija u sve tri domene
života. Sustav CRISPR/Cas9 predstavlja precizan i efikasan alat za ciljano uređivanje genoma i
manipulaciju genske ekspresije. Lakoća programibilnosti ovog dvokomponentnog sustava
promjenom kratke nukleotidne sekvence u molekuli RNA da prepoznaje virtualno bilo koje mjesto
unutar genoma, daje ovoj tehnologiji veliki potencijal za primjenu u genetičkom injženjerstvu,
biotehnologiji te istraživanju, liječenju i prevenciji bolesti uzrokovanih genetičkim ili
epigenetičkim promjenama.

Abstract (english)

Changes in the nucleotide sequence of DNA, as well as epigenetic changes which result in
chromatin perturbation, can lead to development od many diseases in both animals and humans.
Discovery of CRISPR/Cas9 system derived from prokaryotes, in which it serves as an adaptive
immune system, enables determination and investigation od both coding and noncoding DNA,
activation or repression of certain genes and introduction of site-specific mutations in all three
domains of life. CRISPR/Cas9 system is a precise and efficient tool for targeted genome editing
and manipulation of gene expression. Because this two-component system is so easily
programmable by altering short nucleotide sequence of an RNA molecule to target virtually any
locus within the genome, it holds great potential for application in genetic engineering,
biotechnology and investigation, treatment and prevention of diseases caused by genetic or
epigenetic changes.

• Keywords: CRISPR/Cas9
• Language: croatian
• URN:NBN: urn:nbn:hr:217:632140
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-09-09 12:29:44