Bioraspoloživost fenolnih spojeva i bioaktivnost klica kupusnjača u modelu ljudske probave in vitro

master's thesis

• Title (english): Bioavailability of phenolic compounds and bioactivity of Brassicaceae seedlings in in vitro model of human digestion
• Author: Sanja Gagić
• Mentor: Ivana Šola (mentor)
• Committee member: Ivana Šola (predsjednik povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kupusnjače (Brassicaceae) su bogate flavonoidima, glukozinolatima i vitaminima te su zbog
toga jedno od najčešće konzumiranog povrća. Cilj ovog rada bio je usporediti udjele fenolnih
spojeva klica korabice (Brassica oleracea var. acephala gongylodes), kelja (B. oleracea
sabauda), prokulice (B. oleracea gemmifera), cvjetače (B. oleracea botrytis), rotkvice
(Raphanus sativus) i kreše (Lepidium sativum), te njihov antioksidacijski i hipoglikemijski
potencijal nakon in vitro simulirane probave čovjeka kako bi se za svaki od parametara
izdvojilo varijetet klica većeg potencijala. Najbogatije ukupnim (uk.) flavonoidima bile su
klice cvjetače, a uk. hidroksicimetnim kiselinama kelja i rotkvice. Nakon probave u crijevu
najveći udio uk. fenolnih spojeva imale su klice kelja, a uk. flavonoida klice cvjetače.
Najbogatije vitaminom C prije probave bile su klice kreše, a nakon probave u želucu klice
rotkvice. Feruličnom kiselinom najbogatije su bile klice prokulice, a sinapinskom klice kelja i
prije i nakon probave. Najvišu koncentraciju kvercetina prije probave imale su klice cvjetače i
kreše, a nakon probave u crijevima klice cvjetače. Najvišu koncentraciju kempferola i prije i
nakon probave imale su klice rotkvice. Sve klice prije probave pokazale su antioksidacijski
potencijal sličan ili čak i viši od standarda troloksa. Ekstrakti svih klica prije probave pokazali
su isti ili veći stupanj inhibicije α-amilaze nego standardni antidijabetički lijek akarboza.
Nakon probave u crijevu najveći stupanj inhibicije aktivnosti α-amilaze pokazale su korabice.
Prije probave najveći stupanj inhibicije α-glukozidaze pokazale su klice rotkvice, a nakon
probave u crijevu klice korabice. Klice rotkvice, kelja, prokulice i korabice prije probave
snažnije su inhibirale aktivnost α-glukozidaze od akarboze. Zaključujem da klice kupusnjača
predstavljaju biomaterijal velikog potencijala za ljudsku prehranu i zdravlje, te su daljnje
analize njihova biopotencijala itekako potrebne i poželjne.

Abstract (english)

Brassicaceae are rich in flavonoids, glucosinolates and vitamins, and are therefore one of the
most commonly consumed vegetables. The aim of this study was to compare the proportions
of phenolic compounds of kohlrabi (Brassica oleracea var. acephala gongylodes), kale (B.
oleracea sabauda), Brussels sprouts (B. oleracea gemmifera), cauliflower (B. oleracea
botrytis), radish (Raphanus sativus) and cress (Lepidium sativum), and their antioxidant and
hypoglycemic potential after in vitro simulated human digestion in order to single out a
variety of seedlings of higher potential for each of the parameters. The richest in total
flavonoids were cauliflower seedlings, and in hydroxycinnamic acids seedlings of kale and
radish. After intestinal digestion the largest proportion of total phenolics had kale, and total
flavonoids cauliflower seedlings. The richest in vitamin C before digestion were cress
seedlings, and after gastric digestion radish seedlings. Brussels sprouts were the richest in
ferulic acid, and kale seedlings were the richest in synapic acid before and after digestion.
Cauliflower and cress seedlings had the highest concentration of quercetin before digestion,
and cauliflower only after intestinal digestion. Radish seedlings had the highest concentration
of kaempferol both before and after digestion. All pre-digestion seedlings` extracts showed
antioxidant potential similar to or even higher than trolox standard. Extracts of all seedlings
before digestion showed the same or higher degree of α-amylase inhibition than the standard
antidiabetic drug acarbose. After intestinal digestion, the highest degree of inhibition of α-
amylase activity was shown by kohlrabi. Before digestion, the highest degree of α-glucosidase
inhibition was shown by radish seedlings, and after intestinal digestion by kohlrabi seedlings.
Pre-digestion radish, kale, Brussels sprouts and kohlrabi seedlings` extracts inhibited α-
glucosidase activity more strongly than acarbose. Therefore, Brassicaceae seedlings represent
a biomaterial of great potential for human nutrition and health, and further analysis of their
biopotential is very necessary and desirable.

• Keywords: α–amilaza; α-glukozidaza; antioksidansi; fenolne kiseline; flavonoidi; HPLC; specijalizirani metaboliti; spektrofotometrija; vitamin C
• Keywords (english): α –amilase; α-glucosidase; antioxidants; phenolic acids; flavonoids; HPLC; specialized metabolites; spectrophotometry; vitamin C
• Language: croatian
• URN:NBN: urn:nbn:hr:217:963567
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-29
• Created on: 2021-10-14 13:08:10