Odabir fiksativa za procjenu enzimske aktivnosti u citokemijskim testovima leukemija

master's thesis

• Title (english): Selection of fixatives to assess enzyme activity in cytochemical leukemia assays
• Author: Nikolina Dunđer
• Mentor: Maja Šantak (mentor)
• Mentor: Nada Oršolić (sumentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Goran Kovačević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Najčešće fiksirajuće sredstvo u citokemijskim testovima za dijagnostiku leukemija je kombinacija acetona
i formalina. Unatoč svojoj širokoj uporabi, formalin je kancerogen i mutagen, stoga se njegova uporaba
nastoji izbaciti ili svesti na minimum. Trenutno ne postoji adekvatna zamjena formalina, stoga je glavni cilj
ovog rada izraditi probne formulacije fiksativa za citokemijsku dijagnostiku leukemija koji neće sadržavati
formalin i koji neće imati štetno djelovanje, a koji će dati jednako dobre rezultate kao fiksativ s formalinom.
Napravljeno je 36 probnih formulacija, od kojih je 25 sadržavalo manje štetan dialdehid glioksal uz dodatak
suplementa, a ostale nove formulacije nisu uopće sadržavale aldehidne fiksative. Od napravljenih 36
uzoraka njih 8 je fiksiralo stanice usporedivo s fiksativom koji sadrži formalin te su odabrani kao probni
fiksativi u citokemijskim testovima za dijagnostiku leukemija. Šest probnih fiksativa je pokazalo dobre
rezultate pri bojenju kompletima za detekciju leukemija osim bojenja s kompletom Sudan Black B Eco. No
jedna od preostale dvije nove formulacija koje nisu dale dobar rezultat s ostalim testovima za dijagnostiku
leukemija je pokazala dobar rezultat s kompletom Sudan Black B Eco.

Abstract (english)

The most common fixative in cytochemical tests for the diagnosis of leukemia is a combination of acetone
and formalin. Despite its widespread use, formalin is carcinogenic and mutagenic, therefore its use seeks to
be eliminated or minimized. There is currently no adequate replacement for formalin, therefore the main
goal of this paper is to develop trial formulations of fixatives for cytochemical diagnosis of leukemia that
will not contain formalin and that will not have a harmful effect, and which will give just as good results as
a formalin fixative. 36 trial formulations were made, from which 25 contained the less harmful dialdehyde
glyoxal with the addition of a supplement, and the other new formulations did not contain aldehyde fixatives
at all. Of the 36 samples, 8 of them fixed cells comparable to a formalin-containing fixative and were
selected as test fixatives in cytochemical tests for the diagnosis of leukemia. Six trial fixatives showed good
results in staining with leukemia detection kits in addition to staining with the Sudan Black B Eco kit. But
one of the remaining two new formulations that did not give a good result with other tests for the diagnosis
of leukemia showed a good result with the Sudan Black B Eco kit

• Keywords: fiksacija; formalin i enzimi
• Keywords (english): fixation; formalin; enzyme
• Language: croatian
• URN:NBN: urn:nbn:hr:217:079805
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-25 06:47:02