Palinološka analiza i određivanje sadržaja kemijskih elemenata medova sjeverozapadne Hrvatske

master's thesis

• Title (english): Palynological analysis and determination of chemical elements in honeys from Northwestern Croatia
• Author: Tomislav Hudina
• Mentor: Božena Mitić (mentor)
• Mentor: Sanda Rončević (mentor)
• Committee member: Božena Mitić (predsjednik povjerenstva)
• Committee member: Sanda Rončević (član povjerenstva)
• Committee member: Nataša Bauer (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-07-04, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Med je cijenjena, ali još uvijek slabo istražena prehrambena namirnica. Cilj ovog istraživanja bio je dati doprinos poznavanju medova sjeverozapadne Hrvatske. Istraživanje je provedeno na 25 uzoraka medova te je obuhvaćalo palinološku i kemijsku analizu. Palinološka analiza provedena je determinacijom i prebrojavanjem peludnih zrnaca izoliranih iz medova. Kemijska analiza obuhvaćala je spektrometrijsko određivanje sadržaja 17 odabranih kemijskih elemenata u uzorcima medova pomoću atomske emisijske spektrometrije uz induktivno spregnutu plazmu (ICP-AES). Svim su uzorcima snimljeni i ATR (prigušena ukupna refleksija) spektri. Utvrđeno je da samo palinološka analiza nije dovoljna za određivanje botaničkog podrijetla medova, iako je dobra za određivanje geografskog podrijetla. Analiza sadržaja elemenata pokazala se preciznijom u klasifikaciji medova, ali za konačnu potvrdu botaničkog podrijetla ipak je potrebno provesti i palinološku analizu. ATR spektri, samo na temelju asignacije prominentnih vrpci, ne mogu poslužiti za klasifikaciju uzoraka medova, već je potrebna detaljna asignacija.

Abstract (english)

Honey is appreciated, but still poorly investigated nutritional ingredient. The aim of this study was to contribute to the knowledge about honeys from Northwestern Croatia. The study was conducted on 25 samples of honeys and includes palynological and chemical analysis. Pollen analysis was performed by counting and determination of pollen extracted from honey samples. Chemical analysis included the spectrometric determination of 17 selected chemical elements in honey samples using ICP-AES (Inductively Coupled Plasma Atomic Emission Spectrometry) method. In addition, ATR (Attenuated Total Reflection) spectra were recorded to all samples. It turned out that only palynological analysis is insufficient to determine the botanical origin of honey, although it is good for determining the geographic origin. Elements content analysis proved to be more precise for classification of the honeys, but for the final confirmation of botanical origin it is still necessary to implement palynological analysis. ATR spectra can’t be used to classify honey samples just based on the assignment of prominent bands, ie without detailed assignment.

• Keywords: melisopalinologija; ICP-AES; botaničko podrijetlo medova; ATR
• Keywords (english): mellisopalynology; ICP-AES; botanical origin of honey; ATR
• Language: croatian
• URN:NBN: urn:nbn:hr:217:296294
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 08:12:20