Multirezidualna analiza i biogeokemijsko ponašanje opioidnih analgetika u vodenom okolišu

doctoral thesis

• Title (english): Multiresidue analysis and biogeochemical behaviour of opioid analgesics in the aquatic environment
• Author: Petra Kostanjevečki
• Mentor: Senka Terzić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Irena Brčić Karačonji (član povjerenstva)
• Committee member: Vlasta Drevenkar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2021-10-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Opioidni analgetici su psihoaktivne tvari koje nakon primjene dospijevaju u otpadne vode
putem ljudskih izlučevina. U okviru ove disertacije razvijene su analitičke metode za
multirezidualno određivanje tragova 27 terapijskih opioida i njihovih metabolita u otpadnim i
površinskim vodama upotrebom vezanog sustava tekućinske kromatografije i spektrometrije
masa. Razvijene metode primijenjene su za istraživanje njihove rasprostranjenosti i
biogeokemijskog ponašanja u vodenom okolišu. Ukupna koncentracija opioida u otpadnim
vodama Grada Zagreba premašivala je 1 000 ng L-1. Najzastupljeniji predstavnici bili su
tramadol i metadon. Budući da se njihovo uklanjanje u uređajima za pročišćavanje otpadnih
voda pokazalo neučinkovitim, istraženi su alternativni postupci, uključujući biološku
razgradnju adaptiranim mješovitim mikrobnim kulturama i abiotičku razgradnju ozoniranjem.
Ozoniranjem je uklonjeno do 90 % roditeljskih spojeva, a biološkom razgradnjom do 82 % uz
proporcionalno smanjenje toksičnosti za alge. Međutim, i biotičkom i abiotičkom
razgradnjom istraženih spojeva nastala je znatna količina stabilnih transformacijskih
produkata što treba uzeti u obzir kod sveoubuhvatne procjene njihova utjecaja na okoliš.

Abstract (english)

Opioid analgesics are a group of psychoactive substances which enter the aquatic
environment primarily via human excretion. In this thesis, multiresidue analytical methods for
the determination of trace levels of 27 therapeutic opioids in wastewater and surface water
matrices were developed, using liquid chromatography-tandem mass spectrometry. These
methods were applied to study the occurrence and biogeochemical behaviour of the selected
opioids in the aquatic environment. The total concentration of investigated opioids in
wastewater of the city of Zagreb exceeded 1 000 ng L-1, with tramadol and methadone being
the most prominent ones. Due to their rather inefficient elimination in wastewater treatment
plants, two alternative procedures, including biological degradation with enriched mixed
microbal cultures and ozonation were investigated. An improved removal of up to 90% and
82% was achieved in the ozonation and biodegradation experiments, respectively, with a
proportional decrease of toxicity to algae. However, degradation of parent compounds was
associated with the formation of stabile transformation products, which should be taken into
account when assessing the overall impact on the environment.

• Keywords: biološka razgradnja; opioidni analgetici; otpadne vode; ozoniranje; površinske vode; tekućinska kromatografija-spektrometrija masa; transformacijski produkti
• Keywords (english): biodegradation; liquid chromatography-mass spectrometry; opioid analgesics; ozonation; surface water; transformation products; wastewater
• Language: croatian
• URN:NBN: urn:nbn:hr:217:094445
• Promotion: 2021
• Project: Number: IP-2014-09-7031; Title: Sveoubuhvatna procjena ponašanja i sudbine farmaceutski aktivnih tvari u okolišu: makrolidni antibiotici i opioidni analgetici; Title: Comprehensive assessment of the environmental behaviour and fate of pharmaceutically active contaminants: macrolide antibiotics and opioid analgesics; Acronym: COMPASS; Leader: Senka Terzić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: IP-2019-04-9519; Title: Istraživanje farmaceutskog ekspozoma slatkovodnih organizama - procjena rizika i prioritizacija; Title: Investigation of Pharmaceutical Exposome in Freshwater Organisms – Risk Assessment and Prioritization; Acronym: PHARMA-RISK; Leader: Senka Terzić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 162 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-04 16:33:28