Preživljavanje bakterijskih izolata kompleksa Burkholderia cepacia u vodi

master's thesis

• Title (english): Survival of isolates of Burkholderia cepacia complex in water
• Author: Monika Eržen
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Neven Bočić (član povjerenstva)
• Committee member: Vlasta Ćosović (član povjerenstva)
• Committee member: Damir Bucković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-11-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kompleks bakterija Burkholderia cepacia (BCC) skupina je gram-negativnih nefermentativnih
bakterija. Prisutne su u prirodnom okolišu, ali se pojavljuju i kao oportunistički patogen u
imunokompromitiranih bolesnika. Predmet ovog istraživanja bio je utvrditi mogućnost njihova
preživljavanja i eventualnog umnažanja u autoklaviranoj komercijalnoj izvorskoj vodi siromašnoj
i obogaćenoj nutrijentima. Za provedbu istraživanja korištena su četiri okolišna BCC izolata
prethodno izolirana s divljeg odlagališta otpada, sedimenta potoka kod grada Zaboka, vode rijeke
Krapine, poljoprivrednog tla gnojenog gnojem peradi te jedan klinički BCC izolat. Pojedinačni
izolati BCC suspendirani su u vodi siromašnoj i obogaćenoj nutrijentima te su inkubirani na 22 ˚C
tijekom 50 dana. Broj vijabilnih izolata BCC prebrojavan je na početku pokusa i u određenim
vremenskim intervalima te je izračunat postotak preživljavanja svakog izolata. Rezultati su
pokazali da su u vodi s dodatkom nutrijenata svi izolati uspješno preživjeli i umnažali se. U vodi
siromašnoj nutrijentima umnažanje izolata bilo je slabije, ali su bolje preživjeli u odnosu na vodu
obogaćenu nutrijentima. S obzirom da bakterije BCC mogu dugo preživjeti i umnažati se u vodi,
okoliš se treba smatrati glavnim izvorom ovih patogena.

Abstract (english)

The Burkholderia cepacia (BCC) bacterial complex is a group of gram-negative nonfermentative
bacteria. They are present in the natural environment, but also appear as an opportunistic pathogen
in immunocompromised patients. The subject of this research was to determine the possibility of
their survival and eventual multiplication in autoclaved commercial spring water poor and rich in
nutrients. Four environmental BCC isolates previously isolated from wild landfill, stream sediment
near the town of Zabok, Krapina river water, agricultural soil fertilized with poultry manure and
one clinical BCC isolate were used for the research. The individual BCC isolates were suspended
in nutrient-poor and nutrient-rich water and incubated at 22 °C for 50 days. The number of viable
BCC isolates was counted at the beginning of the experiment and at certain time intervals, and the
survival rate of each isolate was calculated. The results showed that in water with the addition of
nutrients, all isolates successfully survived and multiplied. In nutrient-poor water, the
multiplication of isolates was weaker, but they survived better than nutrient-rich water. Since BCC
bacteria can survive and multiply in water for a long time, the environment should be considered
the main source of these pathogens.

• Keywords: Burkholderia cepacia; preživljavanje; voda; okoliš
• Keywords (english): Burkholderia cepacia; survival; water; environment
• Language: croatian
• URN:NBN: urn:nbn:hr:217:020304
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-02 12:03:25