Utjecaj sterigmatocisitna i 5-metoksisterigmatocistina na koncentraciju albumina i aktivnost laktat dehidrogenaze u bronholaveolarnoj tekućini Wistar štakora

master's thesis

• Title (english): Effect of sterigmatocystin and 5-metoxysterigmatocystin on albumin concentration and lactate dehydrogenase activity in bronchoalveolar lavage fluid in Wistar strain rats
• Author: Ivona Molnar
• Mentor: Dubravka Rašić (mentor)
• Mentor: Domagoj Đikić (sumentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Mikotoksini su raznolika skupina sekundarnih metabolita plijesni koji se mogu pronaći u hrani za
ljude i životinje te zraku pljesnivih stambenih prostora. Uzrokuju bolesti (mikotoksikoze)
konzumiranjem kontaminirane hrane, dermalnim putem ili inhalacijom spora. Mikotoksini se
istražuju zbog toksičnih učinaka, gubitaka u poljoprivredi te povezanosti s klimatskim
promjenama. Sterigmatocistin je sekundarni metabolit koji najčešće proizvode plijesni iz roda
Aspergillus. Strukturno je sličan najtoksičnijem karcinogenu u prirodi – aflatoksinu. Toksični
učinci sterigmatocistina nisu dovoljno istraženi, ali je klasificiran kao mogući karcinogen za ljude.
Neke plijesni iz roda Aspergillus često uz sterigmatocistin proizvode 5-metoksisterigmatocistin,
pogotovo u vlažnim stambenim prostorima. Pojam sindrom bolesnih zgrada opisuje skup
simptoma bolesti respiratornog i središnjeg živčanog sustava te se mogu povezati s mikotoksinima.
U ovom su pokusu mužjaci štakora soja Wistar jednokratno tretirani intratrahealnom instilacijom
sterigmatocistinom, 5-metoksisterigmatocistinom i njihovom kombinacijom. U bronhoalveolarnoj
tekućini mjerena je koncentracija albumina kao indikatora propusnosti membrane krvnih žila i
aktivnost laktat dehidrogenaze u svrhu određivanja citotoksičnosti. Povećana koncentracija
albumina i povećana aktivnost lakstat dehidrogenaze ukazuju na toksičnost ovih mikotoksina. Iako
rezultati nisu statistički značajni, važan su doprinos poznavanju toksičnosti ovih mikotoksina
izloženima putem dišnog sustava te ukazuju na potrebu budućih istraživanja o dugotrajnoj
izloženosti sterigmatocistinu i 5-metoksisterigmatocistinu.

Abstract (english)

Mycotoxins are a diverse group of secondary metabolites produced by molds and can be found in
food and feed or air of moldy indoor spaces. They cause diseases (mycotoxicosis) via ingestion of
contaminated food, through skin or inhalation of spores. Mycotoxins are studied for their toxic
effects, because they cause agricultural losses, and in association with climate change.
Sterigmatocystin is a secondary metabolite mostly produced by Aspergillus molds. Structurally it
is similar to the most potent carcinogen found in nature – aflatoxin. Even though the toxic effects
of sterigmatocystin are not well-known, it is classified as possibly carcinogenic to humans. Some
Aspergillus molds often produce 5-metoxysterigmatocystin together with sterigmatocystin,
especially in moldy indoor environments. The term sick building syndrome describes a group of
respiratory and central nervous system disease symptoms that can be related to mycotoxins. In the
present experiment, male Wistar strain rats were intratracheally instilled with a single dose of
sterigmatocystin, 5-metoxisterigmatocystin and their combination. In bronchoalveolar lavage
fluid, the following parameters were measured: concentration of albumin as an indicator of
vascular membrane permeability and lactate dehydrogenase activity as an indicator of cytotoxicity.
Results of acute treatment showed an increase in albumin concentration and increase of lactate
dehydrogenase activity. Even though our results were not statistically significant, they contribute
to the general knowledge on the toxicity of these mycotoxins and they are important for future
research into long-term exposure to mycotoxins.

• Keywords: mikotoksini; plijesni; poplava; sindrom bolesne zgrade; toksičnost
• Keywords (english): : flood; molds; mycotoxins; sick building syndrome; toxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:319896
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-21 13:58:11