Učinak kvercetina na zelenu hidru (Hydra viridissima Pallas, 1766)

master's thesis

• Title (english): The effect of quercetin on green hydra (hydra viridissima Pallas, 1766)
• Author: Ana Matulić
• Mentor: Goran Kovačević (mentor)
• Committee member: Vesna Petrović Peroković (predsjednik povjerenstva)
• Committee member: Goran Kovačević (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-11-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Istraživan je učinak kvercetina u laboratorijskim uvjetima na zelenu hidru Hydra viridissima Pallas i pripadajuću endosimbiotsku algu. Kvercetin je najviše proučavani flavonoid poznat po svojim antioksidativnim svojstvima koja su dobro istražena, no u nekim slučajevima kvercetin može djelovati prooksidativno. Hidre su tretirane s četiri koncentracije kvercetina u trajanju od tri dana i uspoređene s kontrolnom skupinom. Eksperiment je ponavljan tri puta. Upotrebom svjetlosne mikroskopije, morfometrije te makroskopskih opažanja praćene su morfološke i citološko-histološke promjene te promjene u ponašanju zelene hidre, dok je na endosimbiotskoj algi praćena vijabilnost i dioba, oblik i veličina stanica i kloroplasta, razina oštećenja, intenzitet zelene boje kao i povezanost nastalih promjena s koncentracijom toksikanta. Rezultati su pokazali različite morfološke promjene na tijelu hidre kao i promjene u ponašanju: depigmentacije, različite deformacije, migracije, poremećaje u kretanju, sporo reagiranje na mehanički podražaj i mortalitet. Utvrđene su i različite histopatološke promjene na tijelu hidre. Na algama su također nastale različite promjene u obliku i veličini stanica i kloroplasta te intenzitetu zelene boje. Koncentracija kvercetina 0,25 g/L pokazala se prijelaznom koncentracijom prilikom koje su uočene intenzivne subletalne promjene u ponašanju te u morfološkoj i histološkoj strukturi. Koncentracija 0,3 g/L dovela je do ireverzibilnih promjena i gotovo LC50. Na temelju dobivenih rezultata izveden je najvažniji zaključak da kvercetin u navedenim uvjetima pokazuje mogući prooksidativni učinak na zelenu hidru.

Abstract (english)

The effect of quercetin on green hydra Hydra viridissima Pallas and endosymbiotic algae in laboratory conditions was studied. Quercetin is the most studied flavonoid and its the best described property is its ability to act as antioxidant. But in some cases quercetin has prooxidative properties. Green hydras were treated with four concentrations of quercetin for three days and compared to control group of animals. The experiment was performed three times. By the use of light microscopy, morphometry and macroscopic observations morphological, cyto-histological and behavioural changes of green hydra were observed. Cell viability, division, shape and size of cells and chloroplasts, the level of damages and intensity of green colour in relation to the concentration of the toxicant were observed. The results showed distinguished morphological changes on the hydra body as well as behavioural changes: depigmentations, migrations, deformations, disordered locomotion, hardly reacting to the mechanical stimuli and mortality. Also, different histopatological changes on the hydra body were detected. Algae showed changes in size and shape of cells and chloroplast and intensity of green colour. Concentration of 0,25 g/L occured to be treshold concentration in which intensive sublethal and behavioural changes but also changes in morphological and histological structure were detected. Concentration of 0,3 g/L lead to irreversibile changes and ca. LC50. On the basis of the achieved results, the most imporatant conclusion was that quercetin in applied conditions showed possible prooxidative effect on green hydra.

• Keywords: zelena hidra; endosimbiotska alga; kvercetin; toksičnost; morfološke i histološke promjene; morfometrija
• Keywords (english): green hydra; endosymbiotic algae; quercetin; toxicity; morphological and histological changes; morphometry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:536069
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 09:43:23