Genska raznolikost gujavica (Oligochaeta, Annelida) rodova Octodrilus Omodeo, 1956 i Octodriloides Zicsi, 1986 u Hrvatskoj

master's thesis

• Title (english): Genetic diversity of earthworms (Oligochaeta, Annelida) genera Octodrilus Omodeo, 1956 and Octodriloides Zicsi, 1986 in Croatia
• Author: Dino De Bona
• Mentor: Davorka Hackenberger Kutuzović (mentor)
• Mentor: Goran Klobučar (sumentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Committee member: Tajana Begović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Hrvatska je raznolika vrstama gujavica, a teritorij Hrvatske kao dio Balkanskog poluotoka smatra se jednim od centara divergencije vrsta rodova Octodrilus i Octodriloides. Dosad je u Hrvatskoj zabilježeno 15 vrsta roda Octodrilus i 5 vrsta roda Octodriloides. Istraživanje je provedeno u svrhu utvrđivanja genske raznolikosti i raznolikosti vrsta spomenutih rodova DNA barkodiranjem 177 uzoraka s 77 lokaliteta širom Hrvatske. Iz tkiva uzoraka izolirao sam, umnožio i poslao na sekvenciranje sekvence COI regije mtDNA, a upotrebom računalnih programa SEQUENCHER (4.1.4.) i MEGA-X sekvence sam sravnio i obradio. Uz malobrojne, javno dostupne sekvence konstruirao sam filogenetsko stablo prema metodi najveće vjerojatnosti, pomoću kojeg sam izdvojio ukupno 28 linija. Linije sam svrstao u 9 vrsta roda Octodrilus i 3 vrste roda Octodriloides. 3 linije roda Octodrilus zbog specifičnosti u građi ili juvenilnog stadija primjeraka nisu svrstane u opisane vrste. 5 vrsta čini kompleks od najmanje dvije linije sa značajnim genskim udaljenostima. Istraživanje je pokazalo i prednost određivanja vrsta gujavica molekularnim metodama u odnosu na određivanje vrsta prema građi tijela koje je kod gujavica vrlo složeno. Potrebno je provesti istraživanja na daleko većem broju uzoraka kako bi se molekularno-filogenetskim metodama detektirale preostale opisane vrste na području Hrvatske i kako bi se usporedbom s rezultatima ovog istraživanja odredio njihov filogenetski položaj.

Abstract (english)

Croatia is diverse with earthworm species and the Croatian territory, as part of Balkan Peninsula, is considered to be one of the divergency centers of genera Octodrilus and Octodriloides. So far, 15 species of the genera Octodrilus and 5 species of the genera Octodriloides have been recorded in Croatia. The research was conducted in order to determine the genetic and species diversity of the mentioned generas by DNA barcoding 177 samples from 77 localities throughout Croatia. The COI region from mtDNA was isolated, amplified and sequenced from the sample’s tissue. Using the SEQUENCHER (4.1.4) and MEGA-X computer programs, the sequences were aligned and processed and a phylogenetic tree was constructed according to the maximum-likelihood method. A total number of 28 lineages were singled out from a tree and classified to 9 species of the genera Octodrilus and 3 species of the genera Octodriloides. 3 lineages of the genera Octodrilus were not classified to described species due to specificity in structure or juvenile stage of samples. 5 species form a complex of at least two lineages with significant genetic distances. The research also showed the advantage of determining earthworm species by molecular methods instead of determination according to morphology and anatomy, which is very complex. It is necessary to conduct researches on a far larger number of samples in order to detect the remaining described species in Croatia by molecular-phylogenetic methods and to determine their phylogenetic position by comparing them with the results of this research.

• Keywords: rod Octodrilus; rod Octodriloides; filogenija; COI; mtDNA
• Keywords (english): genera Octodrilus; genera Octodriloides; phylogenetics; COI; mtDNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:537570
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-02-23
• Created on: 2022-03-17 18:31:49