Imunohistokemijska vizualizacija proteina SOX2, SOX17 i CALCA u tumoru zametnih stanica sjemenika čovjeka

master's thesis

• Title (english): Immunohistochemical visualization of SOX2, SOX17 and CALCA proteins in human testicular germ cell tumor
• Author: Marina Malnar
• Mentor: Nino Sinčić (mentor)
• Mentor: Romana Gračan (sumentor)
• Committee member: Jasna Lajtner (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-04-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Tumori sjemenika su najčešći tumori koji pogađaju muškarce u najreproduktivnijoj dobi od 15
do 45 godina. Tumori zametnih stanica sjemenika (engl. testicular germ cell tumor, TGCT)
dijagnosticiraju se u oko 95 % slučajeva, što ih čini najčešćim oblikom tumora sjemenika. Kao
vrlo heterogena skupina neoplazija, ugrubo se dijele na seminome i neseminome, a nerijetko se
javljaju u obliku miješanih tumora zametnih stanica. Geni SOX2 i SOX17 su uključeni u
regulaciju diferencijacije embrionalnih stanica i danas se koriste kao tkivni biomarkeri u
dijagnostici embrionalnog karcinoma i seminoma. CALCA je gen supresor tumora, čija je
ekspresija poremećena u nekoliko vrsta tumora. U ovom je istraživanju metodom
imunohistokemije istražena količina i lokalizacija proteina SOX2, SOX17 i CALCA u tkivu
sjemenika ljudi s TGCT-om (N=38), kao i u tkivu sjemenika bez neoplastičnih promjena
(N=20). Protein CALCA je pokazao veliki dijagnostički potencijal za otkrivanje
koriokarcinoma, koji je najagresivniji tip TGCT-a. Koekspresija SOX17 i CALCA, a odsutstvo
proteina SOX2, se pokazala potencijalnim biomarkerom u dijagnosticiranju neoplazije
zametnih stanica in situ koja je preteča zloćudnih TGCT-a.

Abstract (english)

Testicular tumors are the most common type of tumors affecting men in their most reproductive
ages, from 15 to 45 years. Testicular germ cell tumors (TGCTs) are diagnosed in about 95 %
of cases, making them the most common form of testicular tumors. As a very heterogeneus
group of neoplasms, they are roughly divided into seminomas and nonseminomas, and often
occur in the form of mixed germ cell tumors. The SOX2 and SOX17 genes are involved in the
regulation of embryonic cell differentiation and they are used as tissue biomarkers in current
diagnosis of embryonal carcinoma and seminoma. CALCA gene is a tumor suppressor gene
whose expression is disrupted in several types of tumors. In this study, the amount and
localization of SOX2, SOX17 and CALCA proteins were investigated by
immunohistochemical method in testicular tissue of people with TGCT (N=38) and in testicular
tissue without neoplastic changes (N=20). The CALCA protein has shown great diagnostic
potential for detecting choriocarcinoma, which is the most aggressive type of TGCT.
Coexpression of SOX17 and CALCA proteins, with the absence of SOX2 protein, may be used
as a potential biomarkers in the diagnosis of germ cell neoplasia in situ considered as a precursor
of malignant TGCTs.

• Keywords: maligni tumori; protutijelo; tkivni biomarkeri; koekspresija; dijagnostika
• Keywords (english): malignant tumors; antibody; tissue biomarkers; coexpression; diagnostic
• Language: croatian
• URN:NBN: urn:nbn:hr:217:533739
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-04-12
• Created on: 2022-04-28 09:13:00