Funkcionalna analiza metagenoma bioreaktora s djelomičnom reakcijom nitritacije i anaerobne oksidacije amonijaka

master's thesis

• Title (english): Functional metagenomic analysis of a partial nitritation-anammox bioreactor
• Author: Dora Zvjerković
• Mentor: Rosa Karlić (mentor)
• Committee member: Kristian Vlahoviček (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Nenad Malenica (član povjerenstva)
• Committee member: Anamaria Štambuk (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-04-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Otpadne vode prije ispuštanja u okoliš potrebno je obraditi radi uklanjanja tvari koje bi u
prirodnim vodama izazvale ekološki štetnu eutrofikaciju. Za uklanjanje dušikovih spojeva
uglavnom se koriste bioreaktori različitih tipova. U ovom radu korištene su bioinformatičke
metode za taksonomsku i funkcionalnu analizu metagenoma bioreaktora laboratorijske veličine
koji koristi djelomičnu reakciju nitritacije i anaerobne oksidacije amonijaka za uklanjanje
amonijaka iz otpadnih voda koji gotovo potpuno autotrofno, tj. bez potrebe za organskim
ugljikom kao ishranom za aktivne bakterije, uklanja dušik iz otpadnih voda. Pokazano je da je
taksonomski sastav bioreaktora raznolik te da se u njemu nalaze u velikom broju i heterotrofne
skupine vezane uz druge puteve mikrobnog ciklusa dušika, no da su enzimi ciljanih reakcija
(oksidacije amonijaka u nitrit i anammox reakcije) svejedno snažno eksprimirani i da je ova
metoda uklanjanja dušika iz otpadnih voda vjerojatno stabilna i s prisustvom brzo rastućih
heterotrofnih bakterija. Poželjna bi bila slična analiza ovog tipa bioreaktora, na industrijskoj
veličini i pri različitim uvjetima.

Abstract (english)

Removal of various substances from wastewater before it is discharged into the environment is
necessary to ameliorate the harmful ecological effects of eutrophication on natural water bodies.
Different kinds of bioreactors are commonly used for removal of nitrogen from wastewater. In
this master thesis, the taxonomic and functional properties of a laboratory scale partial nitritationanammox bioreactor were analysed. This type of bioreactor removes nitrogen from wastewater
almost completely autotrophically, leaving much of the organic carbon present in influent
wastewater available for anaerobic digestion and biogas production. The analysis showed that the
taxonomic composition of the active microbial community is diverse and contains many
heterotrophic bacterial groups responsible for other pathways of the microbial nitrogen cycle.
Still, enzymes included in nitritation and anammox were predicted to be strongly expressed,
pointing to the stability of the partial nitritation-anammox process even with many fast-growing
heterotrophic species present. In the future, similar analyses should be done on industrial scale
bioreactors operating under variable conditions.

• Keywords: otpadne vode; dušikovi spojevi; bioreaktori
• Language: croatian
• URN:NBN: urn:nbn:hr:217:393684
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-04-28
• Created on: 2022-05-13 10:26:04