Sastav elemenata u otolitima

doctoral thesis

• Title: Sastav elemenata u otolitima : izazovi i mogućnosti za rekonstruiranje ekoloških veza između staništa i vrsta
• Title (english): Elemental composition of otoliths : challenges and opportunities for reconstructing ecological links between habitats and species
• Author: Dario Vrdoljak
• Mentor: Sanja Matić-Skoko (mentor)
• Committee member: Jakov Dulčić (predsjednik povjerenstva)
• Committee member: Melita Peharda Uljević (član povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Geology); Zagreb
• Defense date and country: 2021-10-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Interdisciplinary Natural Sciences; Marine Science
• Universal decimal classification (UDC): 502/504 - Environmental science. Conservation of natural resources. Threats to the environment and protection against them

Abstract

Utvrđeni su elementni (kemijski) sastavi otolita odabranih vrsta riba porodice ljuskavki (Sparidae) pomoću laserske ablacije – induktivno spregnutom plazmatskom masenom spektrometrijom (LA-ICPMS), kristalna struktura otolita komarče, Sparus aurata pomoću konfokalne Ramanove mikrospektroskopije (CRM) te stabilni izotopi u mišićnom tkivu pica, Diplodus puntazzo i fratra, Diplodus vulgaris. Aragonit je glavna komponenta otolita komarče, a orijentacije kristala te tragovi kalcita i stroncijanita potvrdili su zone prirasta. Prikazan je obrazac korištenja estuarijskih i morskih rastilišta te povezanost koncentracije kemijskih elemenata i dijelova otolita. Inovativno kombiniranje elementnih sastava i stabilnih izotopa pokazalo je trofičke razlike kod pica i fratra pri korištenju morskog i estuarijskog rastilišta. Potencijalno je negativna izloženost onečišćenju na ušću Neretve utvrđena kod nedoraslih komarči s individualno povišenim koncentracijama Pb. Korištenjem multivarijatnih i kanonskih analiza uspješno su realocirani pic i fratar u poznata rastilišta na osnovi koncentracija Ba i Sr. Nešto je niža uspješnost postignuta u realokaciji nedoraslih komarči na osnovi koncentracija Sr, Zn i Mg. Daljnji razvoj sklerokronoloških istraživanja, uz utvrđene prepreke i nedostatke, dovest će do njihove šire primjene u Sredozemnome moru i omogućiti bolje razumijevanje ekoloških pokretača promjena u morskom okolišu.

Abstract (english)

The elemental (chemical) compositions of otoliths of selected Sparidae species using Laser ablation – Inductively coupled plasma mass spectrometry (LA-ICPMS), otolith crystal structure of gilthead seabream, Sparus aurata using confocal Raman microscope (CRM) and stable isotope of muscle tissue of sharpsnout seabream, Diplodus puntazzo and common two-banded seabream, Diplodus vulgaris were studied. Aragonite is the main component of seabream otoliths while crystal orientations and calcite and strontianite traces have confirmed growth zones. The pattern of estuarine and marine habitats uses and relationships between the concentration of chemical elements and parts of otoliths are shown. An innovative combination of elemental compositions and stable isotopes indicates trophic differences in D. puntazzo and D. vulgaris when using marine and estuarine habitat. Potentially negative exposure to pollution at Neretva estuary was found in juvenile seabreams with individually elevated Pb concentrations. Using multivariate and canonical analyzes, D. puntazzo and D. vulgaris were successfully reallocated to known habitats based on Ba and Sr concentrations. Slightly lower success was achieved in the reallocation of juvenile seabream based on the concentrations of Sr, Zn and Mg. Further development of sclerochronological research, with identified obstacles and shortcomings, will enable its wider application in the Mediterranean Sea as a basis for a better understanding of the ecological drivers of change in the marine environment.

• Keywords: kemijski sastav otolita; stabilni izotopi; ribe; rastilišta; sklerokronologija
• Keywords (english): otolith chemistry; stable isotopes; fish; nurseries; sclerochronology
• Language: croatian
• URN:NBN: urn:nbn:hr:217:463814
• Promotion: 2022
• Study programme: Title: Interdisciplinary doctoral study in Oceanology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru) (doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru))
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-07-05 11:13:08