Mogućnost ugradnje virusa SARS-CoV-2 u ljudski genom

undergraduate thesis

• Title (english): The possibility of integration of SARS-CoV-2 virus into the human genome
• Author: Sara Lesinger
• Mentor: Ivana Ivančić Baće (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Izvješća o produljenoj detekciji RNA teškog akutnog respiratornog sindroma koronavirusa 2
(SARS-CoV-2) i o kontinuiranim pojavama PCR-pozitivnih testova kod pacijenata mnogo tjedana
nakon oporavka od bolesti COVID-19, dovela su u pitanje mogućnost da se RNA virusa SARSCoV-2 može prepisati reverznom transkripcijom i ugraditi u DNA ljudskih stanica. Zhang i sur.
(2021) istražili su mogućnost bi li transkripcija ugrađenih sekvenci mogla objasniti neke od
pozitivnih PCR testova viđenih kod pacijenata. Otkrili su da se kopije sekvenci DNA SARS-CoV-2
mogu ugraditi u genom zaraženih ljudskih stanica i dokaze koji upućuju na to da se veliki dio
virusnih sekvenci prepisuje iz ugrađenih sekvenci DNA, stvarajući ljudsko-virusne kimerne
transkripte. Parry i sur. (2021) i Smits i sur. (2021.) smatrali su potrebnim pomnije razmotriti
predstavljene dokaze. Pokazali su da su podaci neprikladno interpretirani i ponovno su analizirali
objavljene podatke. Nisu našli dokaze za genomsku ugradnju virusa, unatoč dokazanoj prisutnosti
predloženog mehanizma. Činjenica da se retrotranskripcija i ugradnja SARS-CoV-2 u genom kod
pacijenata događa u značajnoj učestalosti ili čak uopće, ostaje malo vjerojatna.

Abstract (english)

There have been reports about prolonged detection of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) RNA and recurrence of PCR-positive tests in patients many weeks
after recovery from COVID-19. This raised the possibility that SARS-CoV-2 RNAs can be reversetranscribed and integrated into the DNA of human cells. Zhang et al. (2021) investigated possibility
whether transcription of the integrated sequences might account for some of the positive PCR tests
seen in patients. They found that DNA copies of SARS-CoV-2 sequences can be integrated into the
genome of infected human cells and evidence suggesting that a large fraction of the viral sequences
are transcribed from integrated DNA copies, generating viral–host chimeric transcripts. Parry et
al. (2021) and Smits et al. (2021) felt necessary to scrutinize the evidence presented. They showed
that the data has been inappropriately interpreted and they reanalyzed published data. They found
no evidence for genomic integration of the virus, despite demonstrated availability of the proposed
machinery. The fact that retrotranscription and integration of the SARS-CoV-2 genome happens
in patients at any notable frequency, or even at all, remains unlikely.

• Keywords: reverzna transkripcija | ugradnja | LINE1 | sekvenciranje | SARS-CoV-2
• Keywords (english): reverse transcription | integration | LINE1 | sequencing | SARS-CoV-2
• Language: croatian
• URN:NBN: urn:nbn:hr:217:077953
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-04 09:58:37