Antibakterijski učinak eteričnih ulja u "Prirodnim parfemima"

master's thesis

• Title (english): Antibacterial effect of essential oils in "Natural perfumes"
• Author: Ana Gomaz
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-02-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Eterična ulja su sekundarni biljni metaboliti koji se uvelike istražuju kao izvori novih antibakterijskih tvari. Prirodni parfemi mogu biti alkoholne iscrpine eteričnih ulja ili pripravci s baznim uljima. U okviru ovog diplomskog rada ispitan je antibakterijski učinak dva alkoholna i dva uljna prirodna parfema poznatog sastava te njihovih pojedinačnih komponenti (eteričnih ulja pačulija, cedra, tamjana, geranija, ylang-ylanga, lavande, kadulje, lovora, bergamota i naranče) na čiste kulture Gram-negativnih bakterija E.coli i P. aeruginosa, te Gram-pozitivnih bakterija S. aureus i B. subtilis metodom disk difuzije u agaru. Eterično ulje kadulje je imalo najjače djelovanje, na tri ispitana soja (S. aureus, P. aeruginosa i B. subtilis) djelovalo je čak jače od pozitivne kontrole, a dobar antibakterijski učinak pokazali su eterično ulje geranija, bergamota, ylang-ylanga, lovora i lavande. Alkoholna verzija prvog parfema je umjereno djelovala na P. aeruginosa, iako je koncentracija eteričnih ulja bila tek 30%. Hipoteza o mogućem sinergističkom djelovanju eteričnih ulja nije nepobitno potvrđena, ali ni opovrgnuta.

Abstract (english)

Essential oils are secondary plant metabolites and they are screened for new antibacterial substances. Natural perfumes can be prepared as ethanol extracts of essential oils or as mixtures with base oils. This thesis investigates the antibacterial effect of two natural perfumes, prepared as both ethanol and base oil mixtures with defined composition of essential oils, as well as antibacterial effect of utilized essential oils (patchouli, cedarwood, frankincense, geranium, ylang-ylang, lavender, sage, bay, bergamot and orange) on pure cultures of E. coli, P. aeruginosa, S. aureus and B. subtilis by agar disc diffusion method. Sage essential oil had the strongest effect, on three tested strains (S. aureus, P. aeruginosa and B. subtilis) even stronger than positive control, and essential oil of geranium, bergamot, ylang-ylang, bay and lavender showed good antibacterial activity as well. First ethanolic perfume showed moderate effect on P. aeruginosa, although the concentration of essential oils was only 30%. The hypothesis of synergistic action of essential oils is not conclusively confirmed, but neither denied.

• Keywords: bakterija; eterično ulje; prirodni parfem; disk difuzija
• Keywords (english): bacteria; essential oil; natural perfume; disk diffusion
• Language: croatian
• URN:NBN: urn:nbn:hr:217:208550
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 08:20:14