Utjecaj akutne neonatalne hipoksije na razinu katekolamina u moždanom deblu mladih štakora

master's thesis

• Title (english): Effect of acute neonatal hypoxia on the brainstem catecholamine levels in young rats
• Author: Kristina Košić
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Sara Essert (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fetalna hipoksija je stanje koje karakterizira smanjena koncentracija kisika važnog za
normalnu fiziološku funkciju tkiva te normalan razvoj fetusa. Posljedice smanjene koncentracije
kisika očituju se kroz razne neurološke poremećaje kao što su motoričke smetnje, autonomna
disfunkcija, epilepsija, poremećaj pamćenja i pažnje. S obzirom na ulogu katekolamina u
regulaciji adaptivnih ponašanja svojstvenih svim sisavcima, dopaminergički i noradrenergički
neuronski krugovi mogli bi predstavljati mehanizam kojim hipoksija utječe na spomenute
neurološke poremećaje. Cilj ovog rada jest analizirati utjecaj neonatalne izloženosti štakora
kratkotrajnoj akutnoj hipoksiji na koncentraciju katekolamina u moždanom deblu u
adolescentnoj dobi. Koncentracija katekolamina je određena pomoću komercijalnog kompleta
enzimskog imunotesta na čvrstoj fazi (engl. Enzyme-Linked Immunosorbent Assay, ELISA).
Životinje iz hipoksične skupine imale su značajno povišenu koncentraciju dopamina u
moždanom deblu, dok se koncentracija noradrenalina nije promijenila u odnosu na kontrolnu
skupinu. Rezultati istraživanja pokazuju da neonatalna izloženost blagoj prolaznoj hipoksiji
dugoročno narušava homeostazu dopamina.

Abstract (english)

Fetal hypoxia is a condition characterized by a reduced concentration of oxygen
essential for normal physiological tissue function and normal fetal development. The
consequences of the decreased oxygen supply are manifested through various neurological
disorders such as motor disorders, autonomic dysfunction, epilepsy, memory, and attention
disorders. Knowing the role of catecholamines in the inherited adaptive behavioural regulations
of all mammals, dopaminergic and noradrenergic neuronal pathways could represent the
mechanism by which hypoxia affects the aforementioned neurological disorders. This study
aimed to analyze the effect of neonatal exposure to short-term acute hypoxia on the brainstem
catecholamine levels in young rats. Catecholamine concentration has been determined using a
commercial Enzyme-Linked Immunosorbent Assay, ELISA kit. Animals in the hypoxic group had
significantly increased concentrations of the brainstem dopamine, while the concentration of
norepinephrine did not change compared to the control group. The results of the research
show that neonatal exposure to mild transient hypoxia leads to a long-lasting disruption of
dopamine homeostasis.

• Keywords: dopamin; noradrenalin; ELISA; hipokampus; talamus; poremećaji ponašanja
• Keywords (english): dopamine; norepinephrine; ELISA; hippocampus; thalamus; behavioral disorders
• Language: croatian
• URN:NBN: urn:nbn:hr:217:152000
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Title: Brain extracellular matrix in development and in perinatal hypoxia; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Miloš Judaš; Jurisdiction: Croatia; Funder: ERDF
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-28
• Created on: 2022-10-17 09:29:09