Razine katekolamina kod špiljske i potočne populacije kapelske svijetlice (Telestes karsticus)

master's thesis

• Title (english): Catecholamine concentrations in the cave and creek population of the Karst dace (Telestes karsticus)
• Author: Matea Matković
• Mentor: Helena Bilandžija (mentor)
• Mentor: Sofia Ana Blažević (mentor)
• Committee member: Sofia Ana Blažević (predsjednik povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Ivana Šola (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kapelska svijetlica (Telestes karsticus Marčić i Mrakovčić, 2011) stenoendemska je vrsta
dunavskog slijeva koja živi u ponoru i u potoku Sušik. Vrsta Telestes karsticus ima stalnu špiljsku
populaciju, a prisutnost specifične površinske populacije čini je potencijalno važnim modelnim
sustavom za razumijevanje početnih adaptivnih procesa koji nastaju tijekom kolonizacije špilja.
Općenito, ribe koje žive u špiljama razvile su razlike u ponašanju koje su pod kontrolom
katekolamina. Cilj ovog diplomskog rada bio je prilagoditi i validirati postojeću HPLC metodu
mjerenja razine monoamina, izmjeriti koncentracije katekolamina u uzorcima kapelske svijetlice,
utvrditi postoji li razlika u njihovim koncentracijama između špiljskih i potočnih populacija, dolazi
li do promjene u njihovim razinama u eksperimentalnim uvjetima (život u mraku/uvjeti normalnog
dnevno-noćnog ritma) te uzrokuju li spomenuti uvjeti drugačije odgovore kod špiljske i potočne
populacije. Za postizanje navedenog cilja bilo je neophodno pročišćavanje uzoraka ekstrakcijom na
čvrstoj fazi, stoga je razvijena i validirana RP-HPLC metoda za analizu spojeva s katekolnom
skupinom. Špiljska populacija kapelske svijetlice pokazuje indikativno veću koncentraciju
dopamina u mozgu od potočne populacije, što ukazuje na to da bi promjene u neurotransmiterima
mogle imati značajnu ulogu već u inicijalnim koracima naseljavanja podzemlja.

Abstract (english)

Karst Dace (Telestes karsticus Marčić & Mrakovčić, 2011) is a stenoendemic species of the
Danube basin that lives in the Sušik Sinkhole and in the Sušik Creek. Telestes karsticus forms a
permanent cave population and the presence of a specific surface population makes it a potentially
important model system for understanding the initial adaptive processes that occur during the
colonization of caves. In addition, cavefish have evolved differences in behavior which are under
the control of the catecholamine systems. The aims of this thesis was to adapt and validate the
current method for measurement of monoamine concentrations by HPLC, to quantify
catecholamine concentrations in samples of brain and body tissue of the Karst Dace, to determine
whether there is a difference in catecholamine concentrations between cave and surface
populations, to determine whether there is a change in catecholamine concentrations in individuals
exposed to completely dark conditions vs. light/dark conditions and whether these conditions lead to
different responses in cave and surface populations. To achieve this goal, it was necessary to purify
samples by solid phase extraction. Therefore, the RP-HPLC method for the analysis of compounds
containing catechol moiety was developed and validated. The cave population of the Karst Dace
shows a trend of higher concentration of dopamine in the brain compared to the surface population,
indicating that changes in neurotransmitters could play an important role already in the initial
adaptive processes that occur during cave colonization.

• Keywords: HPLC; neurotransmiteri; dopamin; kolonizacija špilja; modelni sustav
• Keywords (english): HPLC; neurotransmitters; dopamine; cave colonization; model system
• Language: croatian
• URN:NBN: urn:nbn:hr:217:968798
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-09-30
• Created on: 2022-10-20 12:45:49