Utjecaj askorbinske kiseline na dinamiku rasta tumorskih sferoida dobivenih iz osteosarkoma

master's thesis

• Title (english): The effect of ascorbic acid on the growth dynamics of osteosarcoma-derived tumor spheroids
• Author: Monika Ranilović
• Mentor: Inga Urlić (mentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Trenutna terapija osteosarkoma sastoji se od kemoterapije i kirurškog odstranjivanja, no nerijetko
dolazi do ponovne pojave bolesti i metastaza. U tome važnu ulogu imaju tumorske matične stanice
koje se iz cjelokupne populacije tumorskih stanica mogu selektivno uzgajati u neprijanjajućim
uvjetima trodimenzionalne kulture. Askorbinska kiselina poznati je antioksidans, no u određenim
uvjetima djeluje i pro-oksidativno. Istraživanja su pokazala da zahvaljujući još nekim svojstvima,
selektivno negativno djeluje na vijabilnost različitih tumorskih stanica. Cilj istraživanja bio je
testirati utjecaj tretmana askorbinskom kiselinom na subpopulaciju matičnih stanica u kulturi za
uzgoj sferoida u prisutnosti i bez prisutnosti FGF2 (fibroblastnog faktora rasta 2). Tijekom 28 dana
pratila sam kako tretman dvjema koncentracijama askorbinske kiseline utječe na broj i veličinu
sferoida u prisutnosti i bez prisutnosti FGF2. Osim toga, testirala sam kako 24-satni tretman
askorbinskom kiselinom utječe na udio apoptotskih stanica u adherentnoj kulturi osteosarkoma.
Rezultati su pokazali kako tretman askorbinskom kiselinom ima negativan učinak na broj i veličinu
sferoida te da prisutnost FGF2 ne sprječava takav učinak. Tretman 10 mM askorbinskom kiselinom
24 sata značajno je povećao udio stanica koje su krenule u apoptozu. Rezultati, dakle, ukazuju kako
askorbinska kiselina ima negativan učinak na matične stanice osteosarkoma neovisno o prisustvu
FGF2.

Abstract (english)

The current treatment of osteosarcoma consists of chemotherapy and surgical removal, but relapse
and metastasis often occur. Here tumor stem cells have an important role. They can be selectively
grown from the population of tumor cells in non-adherent conditions of three-dimensional culture.
Ascorbic acid is a well-known antioxidant, but under certain conditions it acts as a pro-oxidant.
Research has shown that thanks to some other properties, it has a selective negative effect on the
vitality of various tumor cells. The aim of this research was to test the effect of ascorbic acid
treatment on the subpopulation of stem cells in spheroid culture in the presence and without the
presence of FGF2 (fibroblast growth factor 2). Over 28 days, I monitored how treatment with two
concentrations of ascorbic acid affects the number and size of spheroids in the presence and absence
of FGF2. In addition, I tested how a 24-hour treatment with ascorbic acid affects the proportion of
apoptotic cells in an adherent osteosarcoma culture. The results showed that ascorbic acid treatment
has a negative effect on the number and size of spheroids and that the presence of FGF2 does not
interfere with this effect. Treatment with 10 mM ascorbic acid for 24 hours significantly increased
the proportion of cells that started apoptosis. The results, therefore, show that ascorbic acid has a
negative effect on osteosarcoma stem cells and that the effect is independent of FGF2 presence.

• Keywords: vitamin C; sferoidi osteosarkoma; matične stanice; 3D kultura; tretman
• Keywords (english): vitamin C; osteosarcoma spheroids; stem cells; 3D culture; treatment
• Language: croatian
• URN:NBN: urn:nbn:hr:217:578763
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-27
• Created on: 2022-10-20 12:58:24