Antibakterijska aktivnost hidroksiapatita obogaćenog ionima srebra

master's thesis

• Title (english): Antibacterial activity of silver-doped hydroxyapatite
• Author: Helena Turk
• Mentor: Tomislav Ivanković (mentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Hidroksiapatit je obećavajući biomaterijal u regeneraciji kostiju zbog svojih bioaktivnih
svojstva te strukturne i kemijske sličnosti s anorganskom fazom koštanog tkiva. Zbog nedostatka
antibakterijskih svojstva, supstituira se ionom srebra za postizanje antibakterijske aktivnosti.
Srebro posjeduje antibakterijska svojstva, no može biti citotoksično u većim koncentracijama. Za
smanjenje citotoksičnosti srebrova iona, kosupstituira se stroncijevim ionom. Cilj ovog istraživanja
bio je odrediti antibakterijsku aktivnost uzoraka hidroksiapatita obogaćenog ionima srebra i
hidroksiapatita obogaćenog ionima srebra i stroncija metodom disk difuzije i određivanjem
brojnosti bakterija u suspenziji. Testirane bakterije bile su Escherichia coli i Staphylococcus aureus
kao predstavnici najčešćih patogena u infekcijama kostiju. Nesupstituirani hidroksiapatit nije
pokazao antibakterijsku aktivnost. Svi uzorci hidroksiapatita supstituirani srebrovim ionom i uzorci
hidroksiapatita supstituirani srebrovim i stroncijevim ionom pokazali su antibakterijsku aktivnost
u obje metode što je vidljivo iz pojave zone inhibicije i analizom broja živih bakterija u bakterijskoj
suspenziji nakon kontakta s uzorcima u određenom vremenu. Antibakterijska aktivnost uzoraka
hidroksiapatita supstituiranih ionima srebra bila je ovisna o vremenu kontakta i količini
supstituiranog srebrova iona.

Abstract (english)

Hydroxyapatite is a promising biomaterial in bone regeneration due to its bioactive properties
as well as its structural and chemical similarities to the inorganic phase of bone tissue. Due to lack
of antibacterial properties, it is substituted with a silver ion to achieve antibacterial activity. Silver
has antibacterial properties, but can be cytotoxic in higher concentrations. To reduce the
cytotoxicity of silver ions, it is co-substituted with strontium ion. The aim of this study was to
determine the antibacterial activity of samples of hydroxyapatite enriched with silver ions and
hydroxyapatite enriched with silver and strontium ions using the disk diffusion method and
determining the number of bacteria in suspension. The tested bacteria were Escherichia coli and
Staphylococcus aureus as representatives of the most common pathogens in bone infections.
Unsubstituted hydroxyapatite did not show antibacterial activity. All hydroxyapatite samples
substituted with silver ion and hydroxyapatite samples substituted with silver and strontium ions
showed antibacterial activity in both methods, which is evident from the appearance of the
inhibition zone and the analysis of the number of living bacteria in the bacterial suspension after
contact with the samples for a certain amount of time. The antibacterial activity of hydroxyapatite
samples substituted with silver ions was dependent on the contact time and the amount of
substituted silver ions.

• Keywords: antibakterijsko djelovanje; hidroksiapatit; ion srebra; ion stroncija; Staphylococcus aureus; Escherichia coli
• Keywords (english): antibacterial activity; hydroxyapatite; silver ion; strontium ion; Staphylococcus aureus; Escherichia coli
• Language: croatian
• URN:NBN: urn:nbn:hr:217:719437
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-19
• Created on: 2022-10-21 11:08:05