Promjene biokemijskih odrednica u serumu miša nakon primjene pesticida Imazalila i Cipermetrina

master's thesis

• Title (english): Changes of serum biochemical parameters in mice after application of pesticides Imazalil and Cypermethrin
• Author: Goran Lončar
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Maria Špoljar (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-01-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Imazalil i cipermetrin su detektibilni u prehrambenim namirnicama u Hrvatskoj. Cilj je istražiti interaktivni učinak spomenutih pesticida i njihove kombinacije na biokemijske odrednice u Swiss miševa in vivo. Životinje su podijeljene u skupine (6-7 životinja po skupini). Svaka skupina životinja izložena je NOAEL (engl. No observable adverse effect level) dozama pojedinog pesticida te njihovoj kombinaciji (imazalil+cipermetrin, NOAEL 1:1). Pesticidi su svakodnevno aplicirani oralno tijekom 28 dana, nakon čega su utvrđeni serumski biokemijski markeri: LDH (laktat-dehidrogenaza), ALT (alanin-aminotransferaza), AST (aspartat-aminotransferaza), ALP (alkalna fosfataza), GGT (gama-glutamil transferaza), α-amilaza, CK (kreatin-kinaza), kreatin, te ukupni proteini. Pojedinačni pesticidi pokazali su različiti toksični potencijal i to cipermetrin veći toksični potencijal od imazalila, uglavnom povišenjem aktivnosti serumskih enzima. Ova dva pesticida djeluju sinergistički. Pri malim dozama kombinacija potencira njihovo toksično djelovanje i uzrokuje citotoksične biokemijske i patološke promjene u jetri i perifernim tkivima.

Abstract (english)

Imazalil and cypermethrine can be detected in alimetaries in Republic of Croatia. The aim of this research was to explore interactive effect of these pesticides and their combination on biochemical determinants of Swiss mice in vivo. The animals were distributed into groups (6-7 animals/group). Each group was exposed to the NOAEL (No observable adverse effect level) doses of the each individual pesticide and their combination (imazalil+cypermethrine, NOAEL, 1:1). Pesticides were applied orally during 28 days on a daily basis. After that periode the following biochemical parameters were determined: LDH (lactate dehydrogenase), ALT (alanine transaminase), AST (aspartate aminotransferase), ALP (alkaline phosphatase), GGT (gamma glutamyl transferase), α-amilase, CK (creatine-kinase), creatine and total proteins. Individual pesticide indicate different toxic potential (cypermethrin higher toxic potential than imazalil), generally characterized by increase in marker enzyme acitivities. These two pesticides have synergistic effects. Low dose exposure to combination potentiates their individual toxicity to cause subtle biochemical and pathologic changes in liver and peripheral tissue.

• Keywords: pesticidi; cipermetrin; imazalil; enzimi; Swiss-miš; jetra
• Keywords (english): pesticide; Cypermethrine; Imazalil; enzyme; Swiss-mice; liver
• Language: croatian
• URN:NBN: urn:nbn:hr:217:683619
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 09:16:36