Komparativna histomorfometrijska i histokemijska analiza probavnog sustava pet vrsta glodavaca

master's thesis

• Title (english): Comparative histomorphometric and histochemical analysis of gastrointestinal tract of five rodent species
• Author: Mia Jakopović
• Mentor: Duje Lisičić (mentor)
• Mentor: Romana Gračan (komentor)
• Committee member: Duje Lisičić (predsjednik povjerenstva)
• Committee member: Ivana Buj (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Marko Ćaleta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Prehrana životinja odražava se u građi probavnog sustava, a razlike se mogu očitovati u debljini
pojedinih slojeva te broju i tipu žljezdanih vrčastih stanica koje izlučuju mukozni sekret za
probavu i zaštitu. U ovom radu izabrala sam pet vrsta glodavaca koji žive u simpatriji na
krškom području centralne Dalmacije, s ciljem utvrđivanja postoje li histomorfometrijske i
histokemijske razlike u građi probavnog sustava koje su rezultat razlika u prehrani ovih vrsta.
Uključene vrste su Apodemus epimelas, Apodemus sylvaticus, Dinaromys bogdanovi, Eliomys
quercinus i Glis glis. Histomorfometrijski rezultati su pokazali veću debljinu oroženog epitela
jednjaka i manju debljinu vanjskog mišićnog sloja jednjaka kod karnivornih vrsta, dok vrste
koje se hrane visoko kaloričnom i lako probavljivom hranom imaju manju debljinu sluznice
žlijezdanog dijela želuca i deblji epitel debelog crijeva. Vrste kod kojih prehrana sadrži otrovne
tvari imaju specifične prilagodbe debljine pojedinih slojeva. Histokemijska analiza pokazala je
da karnivorne vrste imaju više kiselih vrčastih stanica u tankom crijevu, a granivorne više
mješovitih. Ovi rezultati predstavljaju nove podatke za uključene vrste i daju novi uvid u
postojanje razlika između srodnih vrsta istraživanih glodavaca s različitom prehranom, ali i
između različitih porodica, miševa iz roda Apodemus i puhova, sa sličnom prehranom.

Abstract (english)

Animal diet is reflected in the structure of the digestive tract. Some of the differences can be
manifested in the thickness of individual layers or the number and type of glandular goblet
cells that secrete mucose for digestion and protection. For this thesis, I selected five species of
rodents that live in a sympatry in the karstic area of central Dalmatia, with the aim of
determining if there are any histomorphometric and histochemical differences in the structure
of the gastrointestinal tract, as a result of distinct diets of the species. Species that were included
are Apodemus epimelas, Apodemus sylvaticus, Dinaromys bogdanovi, Eliomys quercinus i Glis
glis. The histomorphometric results showed thicker esophageal keratenized epithelium and
thinner esophageal outer muscular layer in carnovorous species, while species with highcalorie and easily digestible diets have thinner mucose layer of the glandular part of the
stomach and thicker epithelium of the large intestine. Species whose diet contains toxic
subtances showed specific adaptations in the thicknesses of certain layers. Histochemical
analysis showed that carnivorous species have more acidic goblet cells in the small intestine,
while granivorous species have more mixed ones. These results represent new data for included
species and provide new insight into the existence of both differences between related species
of rodents with distinct diets, and between species of different families but with similar diet, as
between Apodemus genus and dormice species.

• Keywords: gastrointestinalni slojevi; jednjak; crijeva; vrčaste stanice
• Keywords (english): gastrointestinal layers; oesophagus; intestines; goblet cells
• Language: croatian
• URN:NBN: urn:nbn:hr:217:563414
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-09-23
• Created on: 2022-10-25 11:00:41