Povezanost alela mikrosatelita unutar regije gena za čimbenik nekroze tumora s akutnim odbacivanjem transplantata jetre

master's thesis

• Title (english): The association of tumor necrosis factor microsatellite polymorphisms and acute liver graft rejection
• Author: Morana Mikloš
• Mentor: Zorana Grubić (mentor)
• Committee member: Zorana Grubić (predsjednik povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-06-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom radu istražena je raznolikost alela lokusa HLA-A, -B i -DRB1 te alela mikrosatelita TNFa, TNFb i TNFd u skupini od 35 parova davatelj-primatelj u programu nesrodne transplantacije jetre, od kojih je 29 bolesnika imalo epizode akutnog odbacivanja, a šest bolesnika nije. Dobiveni rezultati uspoređeni su s kontrolnom skupinom koja je uključivala 200 zdravih nesrodnih ispitanika. Aleli HLA određeni su molekularnom metodom (PCR-SSP, Polymerase Chain Reaction-Sequence Specific Primers), a aleli mikrosatelita TNF metodom PCR-STR (Short Tandem Repeats). Nije uočena statistički značajna razlika u zastupljenosti alela HLA između bolesničke i kontrolne skupine. Utvrđena je statistički značajno povećana zastupljenost alela TNFa5 među bolesnicima u odnosu na kontrolu. Nije uočena razlika u broju nepodudarnosti HLA i TNF između bolesnika s akutnim i bez akutnog odbacivanja jetre.

Abstract (english)

The polymorphism of HLA-A, -B and -DRB1 loci, and TNFa, TNFb and TNFd microsatellites was investigated in this study among 35 donor-recipient pairs in unrelated liver transplantation program. Episodes of acute rejection were observed for 29 patients, while six patients did not demonstrate acute rejection. Obtained results were compared with results of control group (200 healthy, unrelated subjects). HLA specificities were determined using the PCR-SSP (Polymerase Chain Reaction-Sequence Specific Primers) molecular method, while the determination of alleles at TNF microsatellites was performed by PCR-STR (Short Tandem Repeats) method. No statistically significant difference in the distribution of HLA specificities between controls and patients was observed. A statistically significant increase of TNFa5 allele's frequency was shown among patients when compared to controls. No difference in number of HLA and TNF mismatches between patients with and patients without acute liver rejection was observed.

• Keywords: jetra; transplantacija; HLA; cimbenik nekroze tumora; mikrosateliti
• Keywords (english): liver; transplantation; HLA; tumor necrosis factor; microsatellites
• Language: croatian
• URN:NBN: urn:nbn:hr:217:875881
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 09:34:06