Zaštita modelnih membrana flavonoidima u procesu lipidne peroksidacije

doctoral thesis

• Title (english): Protection of model membranes by flavonoids during lipid peroxidation process
• Author: Anja Sadžak
• Mentor: Suzana Šegota (mentor)
• Committee member: Davor Kovačević (predsjednik povjerenstva)
• Committee member: Danijela Bakarić (član povjerenstva)
• Committee member: Nives Galić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2022-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Polinezasićene masne kiseline u sastavu stanične membrane iznimno su podložne lipidnoj peroksidaciji zahvaljujući velikom broju dvostrukih veza. Lipidna peroksidacija podrazumijeva oksidativnu razgradnju lipida induciranu slobodnim radikalima te rezultira oštećenjem membrane. Smatra se da lipidna peroksidacija ima ulogu u razvoju velikog broja bolesti. Flavonoidi, prirodni polifenolni antioksidansi, istražuju se kao potencijalni lijekovi za mnoge bolesti uzrokovane oksidativnim stresom. Međutim, njihova primjenjivost ograničena je slabom topljivošću u vodenim medijima i nestabilnošću u fiziološkim uvjetima. Navedeni problem moguće je riješiti ugradnjom flavonoida u nanonosače. Zahvaljujući specifičnim magnetskim svojstvima, kao nanonosači se posebno ističu superparamagnetske nanočestice. U sklopu ove doktorske disertacije istražena je interakcija lipida i flavonoida te je uočeno da tri strukturno različita flavonoida štite cjelovitost membrane tijekom lipidne peroksidacije. Nadalje, pripravljeni su nanonosači sa superparamagnetskim svojstvima. Pokazano je da upotreba vanjskog magnetskog polja poboljšava otpuštanje flavonoida iz nanočestica.

Abstract (english)

Polyunsaturated fatty acids, which are abundant in cell membranes, are susceptible to lipid peroxidation due to the large number of double bonds. Lipid peroxidation is oxidative degradation of lipids induced by free radicals which results in membrane damage. It has been correlated with numerous diseases. Flavonoids, natural polyphenolic antioxidants, are being investigated as potential therapeutics. However, their applicability is limited by poor solubility in aqueous media and instability under physiological conditions. One possible solution for the aforementioned problem is incorporation of flavonoids into nanocarriers, among which superparamagnetic nanocarriers stand out due to their exceptional magnetic properties. Within this doctoral dissertations, interaction between lipids and flavonoids has been investigated and it has been shown that three structurally different flavonoids exhibit protective behaviour of the membrane upon initiated lipid peroxidation. Moreover, superparamagnetic nanoparticles were prepared and used as nanocarriers. Drug release was improved with use of an external magnetic field.

• Keywords: flavonoidi; lipidna peroksidacija; magnetit; membrana; nanočestice
• Keywords (english): flavonoids; lipid peroxidation; magnetite; membrane; nanoparticles
• Language: croatian
• URN:NBN: urn:nbn:hr:217:317659
• Promotion: 2022
• Project: Number: IP-2016-06-8415; Title: Zaštitni mehanizmi i učinci flavonoida ugrađenih u nanonosače u modelnim membranama i neuronima; Acronym: NanoFlavNeuroProtect; Leader: Suzana Šegota; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 182 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-09-23
• Created on: 2022-10-27 14:51:03