Trofički odnosi pauka u mediteranskim maslinicima i vinogradima analizirani metodom DNA metabarkodiranja

doctoral thesis

• Title (english): Spider trophic interactions in Mediterranean olive groves and vineyards analyzed using DNA metabarcoding
• Author: Barbara Anđelić Dmitrović
• Mentor: Lucija Šerić Jelaska (mentor)
• Committee member: Sunčica Bosak (predsjednik povjerenstva)
• Committee member: Vlatka Čubrić Čurik (član povjerenstva)
• Committee member: Branka Bruvo Mađarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-02-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

U poljoprivrednim ekosustavima pauci su među najvažnijim grabežljivcima i igraju važnu ulogu u
biološkoj kontroli kukaca nametnika na biljnim kulturama. Koriste različite tehnike lova,
uključujući aktivni lov i pasivnu grabežljivost što utječe na složenost njihovih trofičkih odnosa.
Istraživanje složenih trofičkih mreža olakšano je razvojem metoda molekularne biologije u analizi
okolišne DNA. Prilikom analize trofičkih odnosa metodom DNA metabarkodiranja važna je
istraženost faune određenog područja te popunjenost relevantnih baza podataka. Za utvrđivanje
trofičkih interakcija paukova uzorkovanje jedinki paukova i ostale faune beskralježnjaka
provedeno je u maslinicima i vinogradima na području Zadarske županije. DNA barkodiranje
korišteno je za analizu raznolikosti beskralježnjaka, a DNA metabarkodiranje za izgradnju
trofičkih mreža pauka. Analiza raznolikosti potvrdila je brojne vrste jedinstvene za područje
istraživanja. Metabarkodiranje je potvrdilo složene trofičke mreže zajednice pauka sa značajnim
sezonskim varijacijama i varijacijama ovisno o staništu. Osim toga, naši su rezultati otkrili kako se
pauci hrane nametnicima na biljkama, te da tip upravljanja i tehnika lova određuju njihov potencijal
u biološkoj kontroli.

Abstract (english)

In agricultural systems, spiders are among the most important predators and play a vital role in
controlling insect pests. Spiders, as a group, employ a variety of hunting tactics, including active
hunting and passive web-waiting, which affect their food webs.. The advent of molecular biology
techniques for the environmental DNA analysis has facilitated the study of such complex trophic
networks, while knowledge about local fauna and filling the relevant databases with species with
Mediterranean distribution are crucial. A sampling of spider species and other invertebrate species
was done in the olive orchards and vineyards of Zadar County in order to determine the trophic
relationships of spiders. We used DNA barcoding for invertebrate's diversity analysis and DNA
metabarcoding for building spiders' trophic networks. Diversity analysis confirmed that many
species were unique to the study region. DNA metabarcoding confirmed that spiders have a
complex trophic network with significant seasonal and site-dependent variation. Additionally,
results confirmed spiders consume pests, but the pest management type and guild they belong to
determines their biocontrol capability.

• Keywords: biološka kontrola; citokrom c oksidaza; DNA metabarkodiranje; okolišna DNA; pauci; raznolikost; trofičke mreže
• Keywords (english): biological control; cytochrome c oxidase; diversity; DNA metabarcoding; environmental DNA; spiders; trophic networks
• Language: croatian
• URN:NBN: urn:nbn:hr:217:276721
• Promotion: 2023
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-02-22
• Created on: 2023-02-28 13:37:04