Genska struktura i raznolikost roda Chondrostoma (Actinopteri, Leuciscidae) u Hrvatskoj

master's thesis

• Title (english): Genetic structure and diversity of the genus Chondrostoma (Actinopteri, Leuciscidae) in Croatia
• Author: Toni Todorić
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (predsjednik povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-03-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rijeke jadranskog slijeva kratke su i izolirane, što je potaklo alopatrijsku specijaciju i rezultiralo
visokom razinom endemizma. Endemske ribe roda Chondrostoma koje nastanjuju jadranski slijev su
vrste Chondrostoma knerii (Heckel, 1843), u hrvatskim krajevima poznata pod nazivom podustva, i
Chondrostoma phoxinus (Heckel, 1843), poznata kao podbila. Nedostatak podataka o njihovom
životnom ciklusu, ekološkim nišama i nedostatak samih kriterija za određivanje rijetkosti vrsta otežava
njihovu konzervaciju. Cilj je ovog istraživanja odrediti filogenetske odnose i genetsku raznolikost
podustve, podbile i samog roda Chondrostoma. Uzorci tkiva trbušne peraje su korišteni za umnažanje s
tri genska markera (citokrom b, beta-aktin, BMP 4) lančanom reakcijom polimeraze (PCR). Provedena
je filogenetska rekonstrukcija temeljena na sekvencama navedenih markera korištenjem sljedećih
metoda: metoda najveće parsimonije (MP), metoda najveće vjerojatnosti (ML) i metoda susjednog
povezivanja (MJ). Dobivena filogenetska stabla i mreže potvrdili su nekadašnji bliski kontakt hrvatskih
vrsta s talijanskom vrstom Chondrostoma soetta. Rodovi Achondrostoma, Iberochondrostoma i
Pseudochondrostoma relativno su nedavno odvojeni od roda Chondrostoma. Niska genska raznolikost
vrste C. knerii ukazuje na ugroženost ove vrste pod djelovanjem antropogenih čimbenika.

Abstract (english)

The rivers of the Adriatic Basin are short and isolated, which encouraged allopatric speciation and
resulted in high degree of endemism. Endemic species of Chondrostoma genus which inhabit Adriatic
Basin are species Chondrostoma knerii (Heckel, 1843), known under the name Dalmatian nase in
Croatia, and Chondrostoma phoxinus (Heckel, 1843), known as Minnow nase. Lack of data about their
life cycle, ecological niches and lack of criteria for determining rare species makes their conservation
difficult. The aim of this study was to determine phylogenetic relationship and genetic diversity of
Dalmatian nase, Minnow nase and Chondrostoma genus in general. Samples of pelvic fins tissue were
used for multiplication of three genetic markers (cytochrome b, beta-actin and BMP4) using the
polymerase chain reaction (PCR). Phylogenetic reconstruction was done based on sequences of
aforementioned genetic markers using the methods of maximum parsimony, maximum likelihood and
median joining. Resulting phylogenetic trees and networks confirmed close contact of Croatian and
Italian species in the recent past. Achondrostoma, Iberochondrostoma and Pseudochondrostoma gemus
were relatively recently separated from the genus Chondrostoma. Low genetic diversity of D

• Keywords: endemi; taksonomija; filogenija; populacijska genetika; polimorfizam
• Keywords (english): endemic species; taxonomy; phylogeny; population genetics; polymorphism
• Language: croatian
• URN:NBN: urn:nbn:hr:217:267407
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-03-30
• Created on: 2023-04-03 12:23:21