Utjecaj višestrukih stresora na biološke komponente slatkovodnih ekosustava

doctoral thesis

• Title (english): Impact of multiple stressors on the biological components of freshwater ecosystems
• Author: Ivana Grgić
• Mentor: Marko Rožman (mentor)
• Mentor: Ana Previšić (komentor)
• Committee member: Mirela Sertić Perić (predsjednik povjerenstva)
• Committee member: Vlatka Filipović Marijić (član povjerenstva)
• Committee member: Ivana Babić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-05-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Vodeni ekosustavi izloženi su brojnim prirodnim i antropogenim pritiscima. Glavni cilj ovog
istraživanja je praćenje procesa i molekularnih odgovora ključnih sastavnica slatkovodnih
ekosustava prilikom izlaganja višestrukom stresu. U tu svrhu postavljeni su laboratorijski pokusi
na i) tularima i makrofitima izloženima mikroplastici, farmaceuticima i endokrinim modulatorima
te ii) obraštaju izloženom suši i kemijskom onečišćenju. Za istovremenu karakterizaciju stresora i
praćenje molekularnih odgovara organizama razvijena je sveobuhvatna metoda koja omogućuje
detekciju onečišćivala (farmaceutika, endokrinih modulatora i mikroplastike), metabolita i
proteina iz jednog uzorka tkiva. Analizirajući promjene u regulaciji metabolita i ukupnoj količini
proteina, istraživanje na tularima i makrofitima pruža uvid u štetne učinke mikroplastike na vodene
organizme te uspoređuje utjecaj mikroplastike s utjecajima farmaceutika i endokrinih modulatora.
Eksperiment na obraštaju ukazuje na dinamično međudjelovanje mikrobne zajednice i okoliša
prilikom izlaganja stresu uzrokovanom presušivanjem i farmaceuticima. Stres uzrokovan
presušivanjem oblikuje mikrobnu zajednicu, koja pak pod utjecajem onečišćivala mijenja kemijski
sastav okoliša.

Abstract (english)

Freshwater ecosystems are subject to numerous natural and anthropogenic pressures. The main
objective of this research was to investigate the processes and molecular responses of several key
components of freshwater ecosystems while exposed to multiple stressors. To this end, laboratory
experiments were conducted with i) caddisflies and macrophytes exposed to microplastics,
pharmaceuticals and endocrine disruptors, and ii) biofilms exposed to drought and a mixture of
emerging contaminants. A robust method was developed for simultaneous characterisation of
stressors and monitoring the corresponding molecular responses of organisms, allowing the
detection of emerging contaminants (pharmaceuticals, endocrine disrupting compounds and
microplastics), metabolites and proteins from a single tissue sample. By analyzing metabolic and
total protein variations, the experiment on caddisflies and macrophytes provides valuable insights
into adverse effects of microplastics on aquatic organisms and compares the effects of
microplastics with those of pharmaceuticals and endocrine disruptors. The biofilm experiment
shows a dynamic interaction between the microbial community and its environment during
exposure to desiccation and emerging contaminants. Desiccation stress shapes the microbial
community, which in turn, under the influence of emerging contaminants, modulates chemical
composition of the environment.

• Keywords: tulari; mahovina; obraštaj; nova onečišćivala; proteini; metaboliti; antibiotska rezistencija
• Keywords (english): caddisflies; moss; biofilm; emerging contaminants; proteins; metabolites; antibiotic resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:217:476927
• Promotion: 2023
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-05-25
• Created on: 2023-05-31 10:01:49