Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)

master's thesis

• Title (english): The role of reactive oxygen species in response of potato (Solanum tuberosum) to infection with potato spindle tuber viroid (PSTVd)
• Author: Lucija Kumek
• Mentor: Snježana Mihaljević (mentor)
• Mentor: Dunja Leljak-Levanić (komentor)
• Committee member: Dunja Leljak-Levanić (predsjednik povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Nataša Bauer (član povjerenstva)
• Committee member: Nenad Malenica (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Reaktivni kisikovi oblici (ROS) i salicilna kiselina (SA) igraju važnu ulogu u obrambenom
odgovoru biljaka na patogene. U ovom radu istražena je uloga ROS-ova i SA u interakciji biljaka
krumpira (Solanum tuberosum) i viroida vretenastog gomolja krumpira (PSTVd). Kvantificiran je
sadržaj vodikovog peroksida (H2O2) u listovima biljaka inokuliranih viroidom PSTVd i kontrolnih
biljaka linije divljeg tipa i transgenične linije NahG, te je određen učinak infekcije na akumulaciju
H2O2 u različitim vremenskim točkama nakon inokulacije. Istovremeno je analizirana ekspresija
gena uključenih u signalne puteve ROS-ova, uključujući gene za odabrane katalaze, peroksidaze i
proteinske kinaze aktivirane mitogenima (MAPK). Nadalje, uloga SA u interakciji krumpir-PSTVd
istražena je praćenjem učinka egzogenog tretmana s njenim sintetskim analogom, 2,6-
dikloroizonikotinskom kiselinom (INA), na akumulaciju H2O2 i promjene u ekspresiji gena
povezanih s antioksidacijskim odgovorom reguliranim SA-om. Rezultati ukazuju da odgovor
krumpira na infekciju viroidom PSTVd uključuje akumulaciju H2O2 i pokretanje antioksidacijskog
odgovora reguliranog SA-om koji dovodi do transkripcijske aktivacije peroksidaza i kinaza
MAPK. Opažen je pozitivan učinak tretmana s INA-om na imuni odgovor krumpira, posebice u
liniji NahG. Saznanja dobivena ovim istraživanjem pridonose boljem razumijevanju molekularnih
mehanizama uključenih u interakciju biljaka i viroida.

Abstract (english)

Reactive oxygen species (ROS) and salicylic acid (SA) play a key role in plants' defense response
against pathogens. Herein, the role of ROS and SA in the interaction between potato (Solanum
tuberosum) plants and potato spindle tuber viroid (PSTVd) was investigated. The hydrogen
peroxide content (H2O2) in leaves of control and PSTVd-inoculated wild-type and transgenic NahG
plants was quantified at different time points after inoculation, and the effect of infection on H2O2
accumulation was determined. Simultaneously, the expression of genes involved in ROS signaling
pathways, including catalase, peroxidases and mitogen-activated protein kinases (MAPK), was
analyzed. Furthermore, the role of SA in the potato-PSTVd interaction was investigated by
monitoring the effect of exogenous treatment with 2,6-dichloroisonicotinic acid (INA), a synthetic
analog of SA, on H2O2 accumulation and changes in the expression of genes associated with SAregulated antioxidant response. The results indicate that the response of potato plants to PSTVd
infection involves accumulation of H2O2 and the activation of SA-regulated antioxidant responses,
leading to transcriptional activation of peroxidases and MAPK. A positive effect of INA treatment
on potato immunity, particularly in NahG lines was observed. The findings from this study
contribute to a better understanding of the molecular mechanisms underlying plant-pathogen
interactions.

• Keywords: biljni patogeni; oksidativni stres; antioksidacijski odgovor; salicilna kiselina
• Keywords (english): plant pathogens; oxidative stress; antioxidative response; salicylic acid
• Language: croatian
• URN:NBN: urn:nbn:hr:217:452434
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-14
• Created on: 2023-07-26 08:14:47