Effect of cytokine microenvironment on dendritic cell activation

master's thesis

• Title: Utjecaj citokina mikrookoliša na aktivaciju dendritičkih stanica
• Author: Ozren Majstorović
• Mentor: Martin Faldyna (mentor); ISVU: MF089
• Mentor: Petra Korać (mentor)
• Committee member: Petra Korać (predsjednik povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-02-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Dendritičke stanice (DC) su heterogena skupina potentnih, profesionalnih stanica koje predočavaju antigene (APC). Karakteristične su morfologije i jedinstvene sposobnosti izazivanja primarnog imunološkog odgovora. DC prepoznaju i prenose informacije iz okoline do stanica stečene imunosti, a osim navedenih funkcija imaju važnu ulogu u uspostavljanju tolerancije i regulaciji vrste imunološkog odgovora posredovanog T stanicama. DC proizvode citokine i podložne su aktivaciji koja je posredovana citokinima. Put kojim će imunološki odgovor teći ovisi o složenom međudjelovanju stanica imunološkog sustava te velikom broju drugih čimbenika od kojih važnu ulogu ima mikrookoliš u kojem se stanice nalaze. Opći cilj je bio odrediti utjecaj citokina na aktivaciju dendritičkih stanica. Specifični ciljevi bili su: generiranje DC iz krvi svinja, utvrđivanje utjecaja različitih koncentracija IFNγ i IL-4 na aktivaciju DC i analiza ekspresije gena IL-1β, IL-10, IL-12, IL-23 i TGFβ kao pokazatelja tipa imunološkog odgovora koji je pokrenut specifičnim citokinom. Rezultati su pokazali da je proupalni citokin IFNγ aktivirao DC uzrokujući povećanu ekspresiju proupalnih citokina IL-1β, IL-12, IL-23 i inhibiciju ekspresije protuupalnih citokina IL-10 i TGFβ, dok je tretman s protuupalnim citokinom IL-4 pokazivao suprotan učinak. Također je utvrđen i izrazit utjecaj koncentracije citokina na razinu ekspresije, upućujući na učinak ovisan o koncentraciji.

Abstract (english)

Dendritic cells (DC) are a heterogeneous group of potent, professional antigen-presenting cells (APC) with characteristic morphology and unique ability to induce a primary immune response. DCs capture and transfer information from the outside world to the cells of the adaptive immune system, they are important for the induction of tolerance and the regulation of the type of T cell mediated immune response. DCs produce cytokines and are susceptible to cytokine-mediated activation. The direction of the immune response depends on a complex interaction of immune cells and a number of other factors, where microenvironment in which the cells are located plays an important role. The general objective was to investigate the influence of different cytokines on DCs activation. The specific objectives were: to generate DC from porcine blood, to evaluate the effect of different concentrations of cytokine IFNγ and IL-4 on DC activation and to analyze IL-1β, IL-10, IL-12, IL-23 i TGFβ gene expression, as indicators of type of immune response that was induced trough specific cytokine. The results have shown that proinflammatory cytokine IFNγ activated DCs by causing increased expression of proinflammatory cytokines IL-1β, IL-12, IL-23 and inhibiting the expression of anti-inflammatory cytokines IL-10 and TGFβ, whereas treatment with anti-inflammatory cytokine IL-4 showed the opposite effect. It was also demonstrated that cytokine concentration has a distinct role in regulating expression levels trough dose-dependent mechanism.

• Keywords: monocitne dendritičke stanice (MoDC); Th odgovor; qRT-PCR; svinje
• Keywords (english): monocyte-derived dendritic cells (MoDC); Th response; qRT-PCR; pigs
• Language: croatian
• URN:NBN: urn:nbn:hr:217:895038
• Study programme: Title: Eksperimentalna biologija; Study programme type: sveučilišni; Study level: diplomski; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2017-05-08 09:50:07