Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J

master's thesis

• Title (english): The effect of the probiotic strain Lactobacillus sp. and its exopolysaccharides on the inflammatory process in the intestine of C57BL/6J mouse
• Author: Barbara Toljanić
• Mentor: Mirela Baus Lončar (mentor)
• Mentor: Nada Oršolić (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ulcerozni kolitis je kronična upala debelog crijeva koja zahvaća sluznicu. Ulcerozni kolitis
obilježen je smanjenjem tjelesne težine, proljevom, krvavom stolicom, patohistološkim
promjenama na sluznici crijeva te različitim patofiziološkim procesima kao što je kronična upala,
oksidativni stres, stres endoplazmatskog retikuluma. Budući da je jedan od glavnih čimbenika ove
bolesti disbioza, odnosno smanjena raznolikost bakterija u crijevima, danas se kao mogući pristup
liječenja ulceroznog kolitisa istražuje obrada probioticima. Cilj ovog istraživanja bio je utvrditi
kakav utjecaj ima probiotički soj Limosilactobacillus fermentum i njegovi egzopolisaharidi na
dekstran natrij sulfatom (DSS) inducirani kolitis kod miševa soja C57BL/6J. Indeks razvoja kolitisa
praćen je mjerenjem gubitka težine, kozistencije stolice i krvarenja, a posljedice bolesti analizom
hematoloških, histoloških i molekularnih odrednica metodom kvantitativne lančane reakcije
polimerazom (qPCR). Rezultati ukazuju na djelomočno pozitivan učinak probiotičkog soja
Limosilactobacillus fermentum i njegovi egzopolisaharida na DSS-inducirani kolitis miševa soja
C57BL/6J kroz smanjenu razinu upale i krvarenja te regeneraciju tkiva sluznice

Abstract (english)

Ulcerative colitis is a chronic inflammation of the large intestine restricted to the mucosa.
Ulcerative colitis is characterized by weight loss, diarrhea, bloody stools, pathohistological
changes in the intestinal mucosa and various pathophysiological processes such as chronic
inflammation, oxidative stress, and endoplasmic reticulum stress. Since one of the main factors of
this disease is dysbiosis, which is a reduced diversity of bacteria in the gut, today the use of
probiotics is being investigated as a potential approach for treating ulcerative colitis. The aim of
this research was to determine the impact of the probiotic strain Limosilactobacillus fermentum
and its exopolysaccharides on dextran sodium sulfate (DSS) induced colitis in C57BL/6J mouse.
The colitis activity index was monitored through measurements of weight loss, stool consistency,
and bleeding, while the disease outcomes were analyzed using hematological, histological, and
molecular markers through quantitative polymerase chain reaction (qPCR). The results indicate a
partially positive effect of the probiotic strain Limosilactobacillus fermentum and its
exopolysaccharides on DSS-induced colitis in C57BL/6J mice, manifested by reduced
inflammation and bleeding, as well as mucosal tissue regeneration.

• Keywords: probiotici; upala crijeva; TNFα; anemija; dekstran natrij sulfat (DSS)
• Keywords (english): probiotics; intestinal inflammation; TNFα; anemia; dextran sodium sulfate (DSS)
• Language: croatian
• URN:NBN: urn:nbn:hr:217:907408
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-25
• Created on: 2023-10-16 10:19:53