Antimikrobna osjetljivost bakterije Escherichia coli iz hemokultura pacijenata

master's thesis

• Title (english): Antimicrobial susceptibility of Escherichia coli from patients blood cultures
• Author: Vlatka Domjanović
• Mentor: Anita Novak (mentor)
• Mentor: Tomislav Ivanković (sumentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Julija Erhardt (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES

Abstract

Bakterija Escherichia coli dio je crijevnog mikrobioma ljudi i toplokrvnih životinja, ali je i
oportunistički patogen koji uzrokuje crijevne i vancrijevne infekcije. Cilj ovog rada je ispitati
osjetljivost bakterije E. coli na antibiotike iz hemokultura pacijenata iz KBC Split u 2021. g. i
utvrditi učestalost ESBL – producirajućih (engl. extended – spectrum – beta – lactamases) i CRE
– sojeva (engl. carbapenem – resistant – Enterobacteriaceae). Izolati su redom bili najosjetljiviji
na fosfomicin, karbapeneme, aminoglikozide, antibiotike koje sadrže inhibitore beta – laktamaze,
zatim na cefalosporine, florokinolone, sulfonamide, a najrezistentniji bili su na peniciline.
Utvrđeno je da je 12,2% izolata pripadalo ESBL – producirajućim sojevima, dok nijedan izolat
nije pripadao CRE – sojevima. ESBL – producirajući sojevi pokazali su višu stopu rezistencije
na peniciline, cefalosporine, fluorokinolone, sulfonamide i nešto višu stopu rezistencije na
aminoglikozide i antibiotike koji sadrže inhibitore beta – laktamaza, u odnosu na izolate koji ne
proizvode ESBL. ESBL – producirajući sojevi bili su osjetljiviji na antibiotike koji sadrže
inhibitore beta – laktamaza, u odnosu na peniciline i cefalosporine koji ne sadrže inhibitore beta
– laktamaza.

Abstract (english)

Bacterium Escherichia coli is a part of a healthy gut microbiome of humans and warm-blooded
animals. It is also an opportunistic pathogen, which causes intestinal and extra-intestinal
infections. The aim of this thesis was to test antimicrobial susceptibility of E. coli from blood
cultures of patients tested in KBC Split in 2021 and to determine the frequency of ESBL –
producing (extended – spectrum – beta – lactamases) and CRE – strains (carbapenem – resistant
– Enterobacteriaceae). Isolates were the most susceptible to fosfomycin, carbapenems,
aminoglycosides, antibiotics with beta – lactamase inhibitors, cephalosporins, fluoroquinolones,
sulphonamides and finally penicilins. It was determined that 12,2 % of isolates were ESBL –
producing and none of them was CRE. ESBL – producing strains showed a higher percent of
resistance towards penicilins, cephalosporins, fluoroquinolons, sulponamides, slightly higher
percent of resistance towards aminoglycosides and antibiotics with beta – lactamase inhibitors, in
comparison with non-ESBL producing strains. ESBL – producing strains were more susceptible
to antibiotics containing beta – lactamase inhibitors, compared to penicilins and cephalosporins
which do not contain beta – lactamase inhibitors.

• Keywords: ESBL-producirajući sojevi; CRE-sojevi; antibiotik; MDR; rezistenci
• Keywords (english): ESBL – producing strains; CRE – strains; antibiotic; MDR; resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:217:801663
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-19
• Created on: 2023-10-17 11:21:03