Correlation between bioactive matrix mechanics and MCF-10A-derived cell lines phenotypes

master's thesis

• Title (croatian): Korelacija između mehanike bioaktivnog matriksa i fenotipova staničnih linija izvedenih iz stanične linije MCF-10A Correlation between bioactive matrix mechanics and MCF-10A-derived cell lines phenotypes
• Author: Leon Petric
• Mentor: Juho Pokki-Riikonen (mentor)
• Mentor: Inga Urlić (komentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

During the development of a tumor, various changes occur that cause the formation of tumor
tissue. These changes are largely at cellular and extracellular matrix level. As some other
materials, the extracellular matrix has a physical property called viscoelasticity. This property
is defined by the deformation and return of the matrix to its original position during and after
physical stress respectively. In tumor tissues, it is denser, and cells with an invasive phenotype
must overcome movement through a denser and stiffer matrix. In this work, a synthesized
bioactive matrix was used for seeding cells in 3D cultures, the main components of which are
alginate and collagen type 1, and whose viscoelastic properties can be controlled by regulating
the concentration of calcium crosslinker. The goal of this research is to determine whether there
is a significant difference in the observed phenotypic parameters of the same cell line-derived
spheroids seeded in a bioactive matrix of softer and stiffer mechanical properties. This is being
studied for three different cell lines: MCF-10A, MCF-10AT and MCF-DCIS.com. The obtained
data indicate that the cell line MCF-DCIS.com shows the smallest changes in spheroid
phenotype, followed by MCF-10AT and the largest by MCF-10A, which show a difference in
all studied phenotypic parameters for the two matrices of distinct stiffness.

Abstract (croatian)

ijekom razvoja tumora događaju se razne promjene koje rezultiraju formiranjem tumorskog
tkiva. Te su promjene uvelike na razini stanica i ekstracelularnog matriksa. Kao i neki drugi
materijali, ekstracelularni matriks ima specifično fizikalno svojstvo zvano viskoelastičnost. To
je svojstvo definirano deformiranjem i povratkom materijala u njegov prvobitni položaj tijekom
odnosno nakon fizičkog stresa. Kod tumorskih tkiva on je gušći, a stanice invazivnog fenotipa
moraju savladati kretanje kroz gušći i krući ekstracelularni matriks. U ovome radu za
nasađivanje stanica u 3D kulture koristio se sintetizirani bioaktivni matriks za nasađivanje
stanica u 3D kulturama čije su glavne komponente alginat i kolagen tipa 1. Krutost tog matriksa
može se kontrolirati regulacijom koncentracije kalcija. Cilj ovog istraživanja je utvrditi postoji
li značajna razlika u promatranim fenotipskim parametrima istih sferoida izvedenih iz staničnih
linija nasađenih u bioaktivni matriks mekših i krućih mehaničkih svojstava. To se proučava za
tri različite stanične linije: MCF-10A, MCF-10AT i MCF-DCIS.com. Dobiveni podaci ukazuju
na to kako stanična linija MCF-DCIS.com pokazuje najmanje promjene fenotipa sferoida, zatim
MCF-10AT, a zatim MCF-10A koje pokazuju razliku u svim promatranim fenotipskim
parametrima za dvije različite krutosti bioaktivnog matriksa.

• Keywords: Magnetic microrheometry; Rheometry; Breast cancer; Tumor
• Keywords (croatian): Magnetna mikroreometrija; Reologija; Rak dojke; Tumor
• Language: english
• URN:NBN: urn:nbn:hr:217:115857
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-22
• Created on: 2023-10-18 10:20:32