Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata

master's thesis

• Title (english): The role of estrogen and estrogen receptors in the metastatic potential of head and neck tumors
• Author: Jacqueline-Katrin Kranjčević
• Mentor: Petar Ozretić (mentor)
• Committee member: Jasmina Rokov Plavec (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Mirta Rubčić (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Committee member: Ivica Đilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2023-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Muškarci imaju dva do četiri puta veći rizik od razvijanja tumora glave i vrata (TGV) od žena,
neovisno o etiološkom agensu. Stoga se smatra da izloženost estrogenu ženama pruža zaštitu
od nastanka i metastaziranja TGV. Estrogeni svoju funkciju ostvaruju vezanjem za receptore
estrogena, koji se mogu podijeliti na jezgrene (ERα i ERβ) i membranske receptore estrogena
(GPER1 i NaV1.2). Vezanjem estrogena dolazi do aktivacije transkripcije određenih gena i/ili
signalnih kaskada, koje naposlijetku utječu na gensku ekspresiju. Metodom RT-qPCR određen
je ekspresijski profil gena za receptore estrogena u uzorcima zdrave sluznice usne šupljine,
primarnih tumora i metastatskih limfnih čvorova te u staničnoj liniji Detroit 562 nakon tretmana
17β-estradiolom. Pomoću testa zacjeljivanja rana provjerena je migracijska sposobnost stanica
nakon tretmana 17β-estradiolom i tamoksifenom. Rezultati su pokazali da bi ERα i ERβ mogli
imati ulogu u razvoju TGV, ali da tamoksifen vjerojatno nije pogodan oblik liječenja TGV.

Abstract (english)

Men are at a two- to fourfold higher risk of developing head and neck cancers (HNC) than women, regardless of aetiological agent. Therefore, estrogen is considered to provide protection from HNC development and metastasis in women. Estrogens exert their function by binding to estrogen receptors, which can be divided into nuclear (ERα and ERβ) and membrane estrogen receptors (GPER1 and NaV1.2). Estrogen binding activates the transcription of certain genes and/or signaling cascades, which ultimately regulate gene expression. RT-qPCR was used to determine the expression profile of estrogen receptor genes in healthy mucosa, primary tumor and metastatic lymph node samples as well as the cell line Detroit 562 after 17β-estradiol treatment. The wound healing assay was used to study cell migration after 17β-estradiol and tamoxifen treatment. Results showed that ERα and ERβ might be involved in HNC development, but tamoxifen probably isn't a suitable treatment option for HNC.

• Keywords: estrogena; receptori estrogena; karcinom
• Keywords (english): estrogen; estrogen receptors; cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:217:160036
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-29
• Created on: 2023-10-19 13:58:27